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Plasmid#80002PurposemiRFP PAS domain fused to FRBDepositorInsertFRB
UseTagsPAS domain of miRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA
Plasmid#225350Purposepiggyback backbone for non-fluorescent GFP tether and H2B-IRFP expressionDepositorInsertH2B (H2B Human, Synthetic)
UseTagsmiRFP and non-fluorescent GFP (Y67F)-P2AExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-15
Plasmid#119994PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670DepositorInsertmiRFP670
UsePcr templateTagsmiRFP670ExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z15
Plasmid#120058PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with miRFP670DepositorInsertmiRFP670
UsePcr templateTagsmiRFP670ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P15
Plasmid#120026PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670DepositorInsertmiRFP670
UsePcr templateTagsmiRFP670ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRM15
Plasmid#163694Purposehsp70 promoter inducible expression of DHB:mScarlet-P2A-H2B:miRFP670DepositorUseZebrafish expressionTagsmScarlet and miRFP670ExpressionMutationPromoterHSP70Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR.v2.TetON_Cas13d_U6_DR
Plasmid#196727PurposeSortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.DepositorInsertRfxCas13d-T2A-miRFP670nano
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterTREAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC4EN-F1-mGAF709 (NLS-FKBP-mGAF709)
Plasmid#80004PurposemiRFP709 GAF domain fused to FKBP and NLSDepositorInsertFKBP
UseTagsGAF domain of miRFP709 and NLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC4EN-F1-mGAF670 (FKBP-mGAF670)
Plasmid#80003PurposemiRFP670 GAF domain fused to FKBPDepositorInsertFKBP
UseTagsGAF domain of miRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
px552-sg-gria1-HT
Plasmid#187652PurposeContains HaloTag to be inserted into the NTD of Gria1 (via HITI), single guide RNA to target Cas9 to Gria1 under control of the U6 promoter, and miRFP670 under control of human synapsin promoter.DepositorInsertsHaloTag donor sequence
miRFP670
UseAAV and CRISPRTagsN/AExpressionMammalianMutationPromoterSynapsinAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Bxb1_AJMA
Plasmid#183760PurposeBxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703DepositorInsertsASAP2f
jRCaMP1b
miRFP703
ASAP2f
UseTagsLck and NESExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2-Synapse reporters
Plasmid#196899Purposezebrafish expressionDepositorInsertSynapse reporters
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-autophagosome-lysosome reporters
Plasmid#196135Purposezebrafish expressionDepositorInsertautophagosome-lysosome reporters
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-F-actin-microtubule plus ends reporters
Plasmid#196134Purposezebrafish expressionDepositorInsertF-actin-microtubule plus ends reporters
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TripleColour-StAR
Plasmid#222695PurposeCompetition assayDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsTagBFP, miRFP703, dTomatoExpressionMammalianMutationPGK, TagBFP, dTomato without BsmBI cutsitePromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only