-
Plasmid#191337PurposeExpresses the fluorescently labeled plus-end directed KIF13a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertKIF13aMotor-mScarlet-FKBP (KIF13A Human)
UseTagsExpressionMammalianMutationmotor domain aa 1-639PromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet FRT
Plasmid#141111PurposeNatively produces mNeonGreen, in the presence of Flp or Flpe will be recombined to produce mScarlet.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mScarlet-PuroR [M1G]
Plasmid#171809PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]DepositorInsertmScarlet-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV-PGK-Cre-EFS-mScarletSIIN
Plasmid#172434PurposeLentivirus, expresses Cre recombinase and mScarlet-SIINFEKLDepositorInsertsCre
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-mScarlet-I-Cdt1
Plasmid#191100PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertmScarlet-I-Cdt1 (30-120)
UseAAV and Synthetic BiologyTagsmScarlet-I fused to residues 30-120 of human Cdt1…ExpressionMammalianMutationOptimized to human codon usagePromoterCMV enhancer and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-EF1a-NmScarlet-mAID
Plasmid#140617PurposeExpression of OsTIR1 and mScarlet-mAID protein in mammalian cells under the control of EF1a promoterDepositorInsertOsTIR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-mScarlet-MAVS-5'UTR
Plasmid#184639PurposeFluorescent reporter for mammalian cells: Expression of mScarlet with MAVS 5'UTRDepositorInsertmScarlet
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
GW1-mScarlet-Epac-GeNL
Plasmid#207874PurposeMammalian expression of the FRET/BRET cAMP sensorDepositorInsertmScarlet-hEpac1(149-881)-mNeonGreen-NanoLuc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
T1P/pUBQ10(mTurq->mScarlet)
Plasmid#195945PurposePhiC31 target with pUBQ10 switching from mTurq to mScarletDepositorInserttRBCS-mScarlet-attP_PhiC31-pUBQ10-attB_PhiC31-mTurq-tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW mScarletI-T2A-mTurquoise2
Plasmid#118430PurposeProduces equimolar expression of mScarletI and mTq2DepositorInsertmScarletI-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
peAAV-CKII(0.4)-Chronos_eGFP_GP2A_nls_mScarlet
Plasmid#121542PurposeChronos with red nucleus-targeted fluorescence for excitatory neuronsDepositorInsertblue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet
UseAAV and Cre/LoxTagsExpressionMutationPromoterCamKIIAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUASM1:mScarletNLS-SV40pA
Plasmid#155124PurposeTol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUASM1:mScarletNLS
UseZebrafish expressionTagsExpressionMutationPromoterQUASM1-E1bAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
mScarlet-I-T2A-LSS-SGFP2
Plasmid#112946PurposeProduces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRETDepositorInsertmScarlet-I and LSS-SGFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymScarletI-T2A-mTurquoise2
Plasmid#118441PurposeProduces equimolar expression of yeast codon-optimized mScarletI and mTq2DepositorInsertymScarletI-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-CMV-NmScarlet-mAID
Plasmid#140618PurposeExpression of OsTIR1 and mScarlet-mAID protein in DT40 cells under the control of CMV promoterDepositorInsertOsTIR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW mScarlet-T2A-mTurquoise2
Plasmid#118431PurposeProduces equimolar expression of mScarlet and mTq2DepositorInsertmScarlet-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MCP-VP64-2AmScarlet
Plasmid#121963PurposeMammalian expression, Transcription regulation moduleDepositorInsertVP64 module fused with MCP, recruited by MS2 hairpin
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAT9753-BEAR-mScarlet-preedited
Plasmid#162994PurposeBEAR control plasmid with split mScarlet and intact 5' splice siteDepositorInsertmScarlet split with an intron between amino acids 110-111
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hfYFP-TEV-mScarlet-i
Plasmid#191196PurposeFusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInserthfYFP & mScarlet-I fusion
UseTags6xHis and mScarlet-IExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LSSmGFP-TEV-mScarlet-i
Plasmid#191199PurposeFusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInsertLSSmGFP & mScarlet-I fusion
UseTags6xHis and mScarlet-IExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only