Showing: 321 - 340 of 2354 results
-
Plasmid#190166PurposeExpresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.DepositorInsertEB3N-VVDfast-Halo-iLID
UseTags-VVDfast-mClover3-iLIDExpressionMammalianMutation1-189 aa from EB3PromoterAvailabilityAcademic Institutions and Nonprofits only -
Pan-Neurofascin scFv [L11A/41]
Plasmid#206722PurposeMammalian Expression of Pan-Neurofascin scFV. Derived from hybridoma L11A/41 scFv.DepositorInsertPan-Neurofascin (Rattus norvegicus) recombinant scFV (Nfasc Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pIG-837_NY-ESO-1_TCR_tFAS
Plasmid#207500PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInserttFAS, NY-ESO-1_TCR (FAS Human)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual wt p110α/p85α
Plasmid#199302Purposeco-expression of His-tagged p110α wt and Avi-tagged p85α wt proteinsDepositorUseTagsAvi and HisExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFastBac_HT_B_6xHis-TEV-GFP-NBR1
Plasmid#190928PurposePlasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914DepositorInsertNBR1 (NBR1 Human)
UseTagsStrep-TEV-GFPExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
-
pFastBac1-His6-E3(1)-CUbo-TwinStrep
Plasmid#212712PurposeExpression of His6-E3(1)-CUbo-TwinStrep in insect cellsDepositorInsertE3(1)
UseTags6xHis and CUbo-TwinStrepExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-mApple-6xHp
Plasmid#214412PurposeLipid droplet localization of low-affinity NFAST fused to mApple-6xHpDepositorInsertRspA-N
UseTagsmApple-6xHpExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-mApple-mito
Plasmid#214415PurposeMitochondria localization of low-affinity NFAST fused to mApple-mitoDepositorInsertRspA-N
UseTagsmApple-mitoExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
PMP34-mApple-NFAST(low)
Plasmid#214418PurposePeroxisome localization of low-affinity NFAST fused to PMP34-AppleDepositorInsertRspA-N (SLC25A17 )
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Golgi-FAST-IRES-iRFP670
Plasmid#216345PurposeFor testing localization of pFAST and iRFP670 proteins to Golgi complexDepositorInsertST6Gal1_pFAST-IRES-Sys1_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Mito-FAST-IRES-iRFP670
Plasmid#216346PurposeFor testing localization of pFAST and iRFP670 proteins to mitochondriaDepositorInsertCox8_pFAST-IRES-Tomm20_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Peroxisomes-FAST-IRES-iRFP670
Plasmid#216347PurposeFor testing localization of pFAST and iRFP670 proteins to peroxisomesDepositorInsertpFAST_SKL-IRES-iRFP670_PXMP2
UseTagsmyc for FAST protein onlyExpressionMammalianMutationn/aPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCMV-tomm20-ECFP-RspANFAST
Plasmid#216348Purposetargets ECFP-RspANFAST to mitochondrial membraneDepositorInserttomm20-ECFP-RspANFAST
UseTagstomm20ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
hSyn-FAS-DO-ReaChR-mCitrine-WPRE
Plasmid#64035PurposeExpresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructsDepositorInserthSyn-FAS-DO-ReaChR-mCitrine-WPRE
UseAAVTagsReaChR tagged with mCitrineExpressionMammalianMutationPromoterhSynAvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GFP-dynein-2(D1091–Q4307)
Plasmid#64064PurposeCodon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.DepositorInsertCytoplasmic dynein-2 (DYNC2H1 Human)
UseTagsGFP, His tag, and ZZ tagExpressionInsectMutationDeleted amino acids 1-1090, extra C-terminal vali…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual LIC cloning vector (5A)
Plasmid#30121DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His-Flag-Tipin-L195A
Plasmid#23120DepositorInsertTipin (TIPIN Human)
UseTagsFlag- and HisExpressionInsectMutationChanged Leucine 195 to AlaninePromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-EGFP-WPRE-pA
Plasmid#37091PurposeExpresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)DepositorInsertd1eGFP
UseAAV and Cre/Lox; Cre-offTagsExpressionMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-TdTomato-WPRE-pA
Plasmid#37092PurposeExpresses tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)DepositorInsertTdTomato
UseAAV and Cre/Lox; Cre-offTagsExpressionMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only
Showing: 321 - 340 of 2354 results