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pBAD-6xHis-AviTag-ALFAtag-eGFP
Plasmid#195854PurposeBacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.DepositorInsert6xHis-AviTag-ALFAtag-eGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-mTurbo-mito
Plasmid#187177PurposeExpress mitochondira-localized miniTurbo under the control of CAG promoterDepositorInsertmtActA fused with miniTurbo (BirA mutant)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-H2B-mTurbo
Plasmid#187178PurposeExpress histone H2B tagged with miniTurbo under the control of CAG promoterDepositorInserthistone H2B fused with miniTurbo (BirA mutant)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
TurboID-His6_pET21a
Plasmid#107177Purposeexpresses His6-tagged TurboID in bacteriaDepositorInsertTurboID (BirA mutant)
UseTagsExpressionBacterialMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID
Plasmid#209391PurposeTransiently expressing TurboID in plantaDepositorInsert3XHA-TurboID
UseTagsExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pICSL70002
Plasmid#50333DepositorInsertBiotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pGG-TurboID-GFP
Plasmid#209399PurposeTransiently expressing and visualizing the subcellular localization of TurboID in plantaDepositorInsert3XHA-TurboID
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
