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Showing: 321 - 335 of 335 results

  1. pYFAC-CH2

    Plasmid
    #168978
    Purpose
    AMA1 based expression in filamentous fungi pYFAC-pyrG carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    168978-plasmid-map-sequence-id-353766

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  2. pYFAC-CH3

    Plasmid
    #168979
    Purpose
    AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    168979-plasmid-map-sequence-id-350204

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  3. pFGL1252_TetOFF(Hyg)

    Plasmid
    #118992
    Purpose
    TetOFF (promoter-less) cassette with Hygromycin selection
    Depositor
    Naweed Naqvi
    Article
    Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581
    Inserts
    tTA+Tnos
    Hygromycin cassette
    Ttub+PtetO
    Use
    Fungal expression (in magnaporthe oryzae)
    Tags
    Expression
    Mutation
    Codon optimized for Magnaporthe oryzae
    Promoter
    Aspergillus nidulans TrpC promoter, Ustilago mayd…
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    118992-plasmid-map-sequence-id-232118

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  4. pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI

    Plasmid
    #140202
    Purpose
    CRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology; Proof-of-concept te…
    Tags
    Expression
    Mutation
    G174D
    Promoter
    PelcA
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140202-plasmid-map-sequence-id-310118

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  5. pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT

    Plasmid
    #140201
    Purpose
    Fungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Cloning of crrna an…
    Tags
    dLbCas12a crRNA scaffold
    Expression
    Mutation
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140201-plasmid-map-sequence-id-310178

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  6. FB001

    Plasmid
    #119057
    Purpose
    Promoter trpC from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Promoter trpC
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119057-plasmid-map-sequence-id-237151

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  8. pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC

    Plasmid
    #140197
    Purpose
    Episomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Insert
    dLbCas12a-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Expression
    Mutation
    D832A DNase deactivated
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140197-plasmid-map-sequence-id-309708

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  9. pYFAC-CH2-ubi-Y-pyrG

    Plasmid
    #221131
    Purpose
    Expression of heterologous genes under alcohol induction optimised with ubi-Y-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010
    Type
    Empty backbone
    Use
    Synthetic Biology; Expression in aspergillus and …
    Tags
    Expression
    Mutation
    Promoter
    PalcA-T1-PalcS/M-T2-PaldA expression cassette (al…
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    221131-plasmid-map-sequence-id-445434

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  10. pYFAC-CH2-ubi-M-pyrG

    Plasmid
    #221132
    Purpose
    Expression of heterologous genes under alcohol induction optimised with ubi-M-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010
    Type
    Empty backbone
    Use
    Synthetic Biology; Expression in aspergillus and …
    Tags
    Expression
    Mutation
    Promoter
    PalcA-T1-PalcS/M-T2-PaldA expression cassette (al…
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    221132-plasmid-map-sequence-id-445432

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  12. pLM-AMA15.0 Cas9

    Plasmid
    #138944
    Purpose
    Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Expression
    Mutation
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138944-plasmid-map-sequence-id-364383

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  13. pLM-AMA18.0 dCas9-VPR

    Plasmid
    #138945
    Purpose
    Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Expression
    Mutation
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138945-plasmid-map-sequence-id-366628

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  15. pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

    Plasmid
    #140198
    Purpose
    Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Insert
    dLbCas12a(D156R)-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Expression
    Mutation
    D832A DNase deactivated, D156R for improved activ…
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140198-plasmid-map-sequence-id-309713

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Showing: 321 - 335 of 335 results