Skip to main content
Addgene
Showing: 321 - 340 of 2911 results

  1. pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD

    Plasmid
    #196856
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomes
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    AcGFP-Cdk5rap2-CTDdeltaCBD (Cdk5rap2 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Lacking the centrosome-targeting domain (CTD)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196856-plasmid-map-sequence-id-391815

    Enlarge

  2. pBactin-Nedd1-mOrange2-rCM1

    Plasmid
    #196861
    Purpose
    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-rCM1 (Nedd1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196861-plasmid-map-sequence-id-391830

    Enlarge

  3. pBactin-Nedd1-mOrange2-F75A

    Plasmid
    #196862
    Purpose
    Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-F75A (Nedd1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    F75A in the CM1 domain
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196862-plasmid-map-sequence-id-391831

    Enlarge

  4. BAF53b + GFP/p-Actin-IRES

    Plasmid
    #197297
    Purpose
    Plasmid expressing an antigen targeting BAF53b
    Depositor
    Jerry Crabtree
    Insert
    BAF53b (Actl6b Mouse)
    Use
    Tags
    GFP (GFP observed)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    197297-plasmid-map-sequence-id-388114

    Enlarge

  5. pBetaActin-KIF1aMotor-mScarlet-FKBP

    Plasmid
    #191336
    Purpose
    Expresses the fluorescently labeled plus-end directed KIF1a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    KIF1aMotor-mScarlet-FKBP (Kif1a Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    motor domain aa 1-405
    Promoter
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191336-plasmid-map-sequence-id-381761

    Enlarge

  6. pBetaActin-FKBP-Halo-Dync1h1motor

    Plasmid
    #191333
    Purpose
    Expresses the fluorescently labeled dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-Halo-Dync1h1motor (Dync1h1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    DYNC1H1 motor domain aa 1453-4644
    Promoter
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191333-plasmid-map-sequence-id-381754

    Enlarge

  8. pBetaActin-FKBP-mScarlet-KIFC1motorN593K

    Plasmid
    #191335
    Purpose
    Expresses the fluorescently labeled minus-end directed KIFC1 motor-deficient motor domain fused to FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-mScarlet-KIFC1N593K (KIFC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    motor domain aa 125-673; N593K
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191335-plasmid-map-sequence-id-381757

    Enlarge

  9. pBetaActin-FKBP-mScarlet-KIFC1motor

    Plasmid
    #191334
    Purpose
    Expresses the fluorescently labeled minus-end directed KIFC1 motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-mScarlet-KIFC1 (KIFC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    motor domain aa 125-673; N593K
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191334-plasmid-map-sequence-id-381755

    Enlarge

  10. pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T

    Plasmid
    #191331
    Purpose
    Expresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    DYNC1H1 motor domain aa 1453-4644
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191331-plasmid-map-sequence-id-381751

    Enlarge

  12. Actin Binding Domain Tension Sensor

    Plasmid
    #215368
    Purpose
    Expresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.
    Depositor
    Brenton Hoffman
    Article
    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081
    Insert
    Actin Binding Domain Tension Sensor
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    215368-plasmid-map-sequence-id-430713

    Enlarge

  13. Actin Binding Domain Tensionless Control

    Plasmid
    #215371
    Purpose
    Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).
    Depositor
    Brenton Hoffman
    Article
    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081
    Insert
    Actin Binding Domain Tensionless Control
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    215371-plasmid-map-sequence-id-430717

    Enlarge

Showing: 321 - 340 of 2911 results