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Plasmid#98799PurposeExpression VectorDepositorInsertpET28 MBP-HPLVGHM-TEV L5B 111215
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP968
Plasmid#98800PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQM-TEV H28L T30A
UseTagsExpressionBacterialMutationH28L, T30APromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGST-HsSyx-375-792
Plasmid#179292PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + GST + TEV protease cleavage siteExpressionBacterialMutationresidues 375-792 from accession number NP_0010361…PromoterT7Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-HsSyx3
Plasmid#179288PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + SUMO + TEV protease cleavage siteExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK573
Plasmid#72456PurposeProduces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic geneDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationsynthetic gene with ~57 silent substitutionsPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSRK2721
Plasmid#78572PurposeGateway destination vector for production of MBP-3C site-His6-TEV site-passenger protein fusionsDepositorInsertGateway cloning cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSP577
Plasmid#98801PurposeExpression VectorDepositorInsertpET28 MBP-HNLYFQS-TEV I138T, N171D, N176T
UseTagsExpressionBacterialMutationI138T, N171D, N176TPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP971
Plasmid#98805PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQS-TEV E107D, D127A, S135F, R203Q, K215E
UseTagsExpressionBacterialMutationE107D, D127A, S135F, R203Q, K215EPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP970
Plasmid#98804PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQS-TEV E107D, D127A, S135F
UseTagsExpressionBacterialMutationE107D, D127A, S135FPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP969
Plasmid#98802PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFHS-TEV T146S, D148P, S153N, S170A, N177M
UseTagsExpressionBacterialMutationT146S, D148P, S153N, S170A, N177MPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP824
Plasmid#98803PurposeExpression VectorDepositorInsertpET28 MBP-ENLYGQS-TEV V209M, W211I, M218F
UseTagsExpressionBacterialMutationV209M, W211I, M218FPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK569
Plasmid#72455PurposeProduces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic geneDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationsynthetic gene with ~51 silent substitutionsPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
scFLARE2
Plasmid#158700PurposeNeuronal expression of scFLARE2 componentDepositorInsertscFLARE2
UseAAVTagsHA, Flag, VP16ExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aci…PromoterSynAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
scFLARE1
Plasmid#158738PurposeMammalian expression of scFLARE1 componentDepositorInsertscFLARE1
UseAAVTagsFlag, V5ExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aci…PromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMBP-HsSyx3
Plasmid#179287PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + mature maltose binding protein + TEV prote…ExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMBP-HsSyx-375-792
Plasmid#179291PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + mature maltose binding protein + TEV prote…ExpressionBacterialMutationresidues 375-792 from accession number NP_0010361…PromoterT7Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSP972
Plasmid#98806PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQS-TEV T17S, N68D, E107D, D127A, F132L, S135F, F162S, K229E
UseTagsExpressionBacterialMutationT17S, N68D, E107D, D127A, F132L, S135F, F162S, K2…PromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMLLAC-J72041
Plasmid#26186DepositorInsertTev Protease Cleavage site
UseSynthetic Biology; 2ab assembly vectorTagsExpressionMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCsy_complex
Plasmid#89232PurposePlasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage siteDepositorInsertsCsy1
Csy2
Csy3
Csy4
UseTags6xHis-TEV proteaseExpressionMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-BB0323-ss
Plasmid#137067PurposeE. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66700.1
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature BB0323: lacks the BB0323 signal peptidePromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only