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Showing: 321 - 340 of 16252 results

  2. pRS405 SEC66-VAC8-GFP

    Plasmid
    #166840
    Purpose
    Expresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554
    Insert
    VAC8 (VAC8, SEC66 Budding Yeast)
    Use
    Tags
    GFP and SEC66 transmembrane domain
    Expression
    Yeast
    Mutation
    SEC66 transmembrane domain
    Promoter
    VAC8 promoter
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166840-plasmid-map-sequence-id-329328

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  5. pCAGGS_m-AKAR5

    Plasmid
    #114139
    Purpose
    Mammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    AKAR5 C-terminally tagged by KRas C tail
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    114139-plasmid-map-sequence-id-215468

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  7. pGEX6p1-GST-Rnf31

    Plasmid
    #63134
    Purpose
    Expression of human RNF31/HOIP codon optimized for E. coli and insect cells
    Depositor
    Titia Sixma
    Article
    Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:
    Insert
    Rnf31 (RNF31 Human, Synthetic)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Synthetic based on human protein sequence
    Promoter
    tac
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. EGFP-GRAM-H

    Plasmid
    #211700
    Purpose
    Expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187L mutation (EGFP-GRAM1b G187L) in mammalian cells
    Depositor
    Yasunori Saheki
    Article
    Ercan et al EMBO J. 2021 Mar 15;40(6):e106524. doi:
    Insert
    GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Leucine
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211700-plasmid-map-sequence-id-419222

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  16. pKTop::sptse5-CT (1169-1229)

    Plasmid
    #192948
    Purpose
    For membrane insertion study; expresses Tse5-CT (1169-1229)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus
    Depositor
    David Albesa-Jové
    Article
    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:
    Insert
    sptse5-CT (1169-1229)
    Use
    Tags
    PelB signal peptide
    Expression
    Bacterial
    Mutation
    encodes for tse5 (1169-1229)
    Promoter
    Available since
    Dec. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192948-plasmid-map-sequence-id-380616

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Showing: 321 - 340 of 16252 results