-
Plasmid#199051PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpBBR1-UP ORI plus NGS barcode 21
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
P4 cosmid
Plasmid#196335PurposeP4 cosmid with CRISPR-Cas9 systemDepositorInsertgenes from phage P4, CRISPR-Cas9 system
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_167
Plasmid#199099PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpBBR1 ORI plus NGS barcode 13, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBx-Spas-sgRNA-Kan
Plasmid#105233PurposePlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescensDepositorInsertS. pasteurianus sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL2_173
Plasmid#199105PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpBBR1-UP ORI plus NGS barcode 21, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBx-Spas-sgRNA-Gm
Plasmid#105232PurposePlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosaDepositorInsertS. pasteurianus sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pS3
Plasmid#50596PurposeBackbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5DepositorInsertsaroE
aroD
aroB
aroG*
ppsA
tktA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
Sc eRF1 (WT)
Plasmid#175413PurposeEncodes His-MBP-TEV-WT eRF1DepositorInserteRF1 (SUP45 Budding Yeast)
UseTagsHis-MBP-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOGG024
Plasmid#113991PurposeLevel 1 golden gate cloning vector for medium copy number free-living expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
M2660 ura3::LYS2 Disruptor Converter
Plasmid#51678PurposeYeast marker swap plasmid for converting URA3 to LYS2DepositorInsertLYS2 (LYS2 Budding Yeast)
UseYeast marker swapTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUPD-pTEF2 (01-11)
Plasmid#101685PurposepTEF2 in domestication vectorDepositorInsertGGAG-[promoter of S. cerevisiae YBR118W (520bp)]-CCAT
UseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBA1032
Plasmid#189580PurposeT4soc deletion HR vector 250bp HR Arms (T4 phage editing)DepositorInsertT4soc Deletion Locus 250bp Homology Arms
UseUnspecifiedTagsExpressionMutationEncodes for a full deletion of T4socPromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
BRPF1 (3L42)
Plasmid#25339PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBRPF1_P1 (BRPF1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
D771 leu2::LYS2 Disruptor Converter
Plasmid#51677PurposeYeast marker swap plasmid for converting LEU2 to LYS2DepositorInsertLYS2 (LYS2 Budding Yeast)
UseYeast marker swapTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBA1015
Plasmid#189589PurposeT7gp1.7 deletion HR vector 250bp HR Arms (T7 phage editing)DepositorInsertT7gp1.7 Locus 250bp Homology Arms
UseSynthetic BiologyTagsExpressionBacterialMutationEncodes for a full deletion of T7gp1.7PromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PEP119T
Plasmid#98251PurposePseudomonas T3SS gateway vector with HA-sfGFP11-sfGFP11DepositorInsertGateway cassettee-HA-sfGFP11-11
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJUMP43-2A(sfGFP)
Plasmid#126987PurposeLevel 2 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP23-1A(sfGFP)
Plasmid#126970PurposeLevel 1 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
D1433 his3::LYS2 Disruptor Converter
Plasmid#51676PurposeYeast marker swap plasmid for converting HIS3 to LYS2DepositorInsertLYS2 (LYS2 Budding Yeast)
UseYeast marker swapTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
BRPF1 (3MO8)
Plasmid#28107PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBRPF1:JMC014-E01:C44383 (BRPF1 Human)
UseTagsHis-tag with integrated TEV protease siteExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits