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Plasmid#63914PurposeRecipient overexpressing vector to construct YFP tagged fusionsDepositorTypeEmpty backboneUseTagsYFPExpressionYeastMutationPromoterTEF1 (Saccharomyces cerevisiae)Available sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only
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pWR90
Plasmid#203914Purposeempty BLINCAR plasmid for payload delivery, no payload, integrates at GSC2 locusDepositorInsertGSC2 plus flanking (GSC2 S. stipitis (yeast))
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKE012
Plasmid#207914PurposeLevel 0 for gRNA in AD2 module (5' TGAC-3' TCCC)DepositorInsertpU3-AarI-scaffold-term
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC73-P1G1
Plasmid#202914PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC73DepositorInsertEGFP/BC73
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4EHP_AH
Plasmid#148914PurposeInsect Expression of Dm4EHPDepositorInsertDm4EHP (4EHP Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA EYFP_zhang2.0
Plasmid#167914PurposeLentiviral vector for expressing U6 MS2-sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTK234_039
Plasmid#123914PurposeEncodes the spCas9 tagBFP gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA1 tagBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBDuet140
Plasmid#121914PurposeOrthogonal NpuDnaB mini-intein inteinDepositorInsertOth-NpuDnaBΔ290 intein
UseTagsGB1 and His6-GB1ExpressionBacterialMutationDeletion of 290-residue endonuclease domain, I53K…PromoterT7Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-572.sensor.PGK.dNGFR.WPRE
Plasmid#85914PurposeSensor PlasmidDepositorInsertmiR-572 Sensor (MIR572 Human)
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
PCx35CT-S276A, S298ter
Plasmid#42914DepositorInsertConnexin 35 C-terminal domain
UseTags6x His, GST, and S-tagExpressionBacterialMutationSer276 to Ala and Ser298 stop codonPromoterT7Available sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 BRGI-Flag
Plasmid#19143DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1179 U6-reci Gag-Cas9 v2
Plasmid#201914PurposeCas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-Cas9 v2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCh-alpha-tubulin
Plasmid#49149Purposeexpression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.DepositorInsertalpha tubulin (TUBA1B Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70
Plasmid#62914PurposeCreating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1DepositorInsertT2A-p65AD-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
UAS:mCherry
Plasmid#170994PurposeUAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.DepositorInsertUAS:mCherry
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterSynthetic 14x UAS:Gal4Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHrD-IRES-Fluc
Plasmid#194250PurposeHuman RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterhuman rDNA promoter and beginning of 5' ETS …Available sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF6-myc-TBC1D15
Plasmid#79148Purposemammalian expression of TBC1D15DepositorInsertTBC1D15 (Tbc1d15 Mouse)
UseTagsHis, Myc, and V5ExpressionMammalianMutationS616N (please see depositor comment below)PromoterEF-1alphaAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Dvl2-GFP
Plasmid#109148PurposeStable Dvl2 expressionDepositorInsertDvl2 (DVL2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterSV40Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-InPAkt
Plasmid#181914PurposeIndicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.DepositorInsertInPAkt
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-hDicer
Plasmid#89144PurposeExpression of hDicer in Sf9 CellsDepositorInserthDicer (DICER1 Human)
UseTagsHis6-tevExpressionInsectMutationPromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only