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  2. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA

    Plasmid
    #136657
    Purpose
    Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136657-plasmid-map-sequence-id-273344

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  3. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA

    Plasmid
    #136654
    Purpose
    Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:CNRZ1066 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136654-plasmid-map-sequence-id-273340

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  7. Anti-Kv2.2 K+ channel [N372/1R]

    Plasmid
    #225391
    Purpose
    Mammalian Expression Plasmid of anti-Kv2.2 K+ channel (Rat) IgG2a R-mAb. Derived from hybridoma N372/1.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Kv2.2 K+ channel (Rattus norvegicus) recombinant (Mouse) monoclonal antibody. (Kcnb2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    225391-plasmid-map-sequence-id-449236

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  8. Anti-Kv2.2 K+ channel [N372/46R]

    Plasmid
    #225392
    Purpose
    Mammalian Expression Plasmid of anti-Kv2.2 K+ channel (Rat) IgG2a R-mAb. Derived from hybridoma N372/46.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Kv2.2 K+ channel (Rattus norvegicus) recombinant (Mouse) monoclonal antibody. (Kcnb2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    225392-plasmid-map-sequence-id-449139

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  9. Anti-Kv2.2 K+ channel [N372A/21R]

    Plasmid
    #219465
    Purpose
    Mammalian Expression Plasmid of anti-Kv2.2 K+ channel (Rat) IgG2a R-mAb. Derived from hybridoma N372A/21.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Kv2.2 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody. (Kcnb2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219465-plasmid-map-sequence-id-431259

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  12. YFP(159-238)-gamma-11 in pcDNAI/Amp

    Plasmid
    #55193
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-11. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    YFP(159-238)-gamma-11 (GNG11 Bovine, Aequorea victoria)
    Use
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-11 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55193-plasmid-map-sequence-id-115059

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  13. CFP(1-158)-gamma-11 in pcDNAI/Amp

    Plasmid
    #55620
    Purpose
    An amino-terminal CFP fragment was fused to Ggamma-11. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    CFP(1-158)-gamma-11 (GNG11 Bovine, Aequorea victoria)
    Use
    Tags
    CFP(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-11 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55620-plasmid-map-sequence-id-115163

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  14. Cer(1-158)-gamma-11 in pcDNAI/Amp

    Plasmid
    #55628
    Purpose
    An N-terminal mCerulean fragment was fused to Ggamma-11. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    Cer(1-158)-gamma-11 (GNG11 Bovine, Aequorea victoria)
    Use
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-11 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55628-plasmid-map-sequence-id-115186

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  15. pBS ZfHoxb3a

    Plasmid
    #27522
    Depositor
    Victoria Prince
    Insert
    Hoxb3a (hoxb3a Zebrafish)
    Use
    Riboprobe synthesis
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 24, 2011
    Availability
    Academic Institutions and Nonprofits only

  16. pLV-Enh eGFP Reporter-muKC2-X31

    Plasmid
    #59255
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Krt80-ex5-7
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pZBTB11-donor

    Plasmid
    #112348
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB11 with GFP
    Depositor
    Kevin White
    Insert
    ZBTB11 homology arms flanking EGFP-IRES-Neo cassette (ZBTB11 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:3:101651181:A>T
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112348-plasmid-map-sequence-id-229661

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  18. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201152
    Purpose
    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    dCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mutation
    gRNA sequence: TACGTTCTCTATCACTGAT
    Promoter
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201152-plasmid-map-sequence-id-401799

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  20. pVP91A-pcaHG

    Plasmid
    #113766
    Purpose
    Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.
    Depositor
    John Lipscomb
    Article
    Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11
    Inserts
    β subunit of Protocatechuate 3,4- Dioxygenase
    α subunit of Protocatechuate 3,4- Dioxygenase
    Use
    Tags
    Met-6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T5 promoter
    Available since
    Aug. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    113766-plasmid-map-sequence-id-218346

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Showing: 321 - 340 of 409 results