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Plasmid#159363PurposeMammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.DepositorInsertSARS-CoV-2 S protein (S SARS-CoV-2)
UseTagsFlag and FlagExpressionMammalianMutationFurin-cleavage site mutationPromoterCMVAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-G614 (NC-flag)
Plasmid#156419PurposeMammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorInsertSARS-CoV-2 S protein (S SARS CoV2)
UseTagsFlagExpressionMammalianMutationD614GPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLN466 (FuGW-S(cMYC)p-mKate2)
Plasmid#105189Purposesynthetic promoter cMYC drives mKate2 expressionDepositorInsertS(cMYC)p-mKate2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLN351 (FuGW-S(YBX1)p-mKate2 )
Plasmid#105174Purposesynthetic promoter YBX1 drives mKate2 expressionDepositorInsertS(YBX1)p-mKate2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLN352 (FuGW-S(Elk1)p-mKate2)
Plasmid#105175Purposesynthetic promoter Elk1 drives mKate2 experssionDepositorInsertS(Elk1)p-mKate2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLN380 (FuGW-S(CEBPB)p-mKate2)
Plasmid#105178Purposesynthetic promoter CEBPB drives mKate2 expressionDepositorInsertS(CEBPB)p-mKate2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN381 (FuGW-S(NFY)p-mKate2)
Plasmid#105179Purposesynthetic promoter NFY drives mKate2 expressionDepositorInsertS(NFY)p-mKate2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLN593 (FuGW-S(MAFK)p-mKate2)
Plasmid#105205Purposesynthetic promoter MAFK drives mKate2 expressionDepositorInsertS(MAFK)p-mKate2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 SARS-CoV-2 S-frag5_nostop
Plasmid#153177PurposeGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS-frag5
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pαH-S-GSAS-B.1.351.v2
Plasmid#173786PurposeMammalian expression of SARS-CoV-2 Spike protein South African variant version 2DepositorInsertSpike (S-GSAS-B.1.351 variant version 2) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLN397 (FuGW-S(E2F1)p-Sponge7)
Plasmid#105181PurposeModule 2 - synthetic promoter E2F1 drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)DepositorInsertS(E2F1)p-Sponge7
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 SARS-CoV-2 S-frag3_nostop
Plasmid#153175PurposeGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS-frag3
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221 SARS-CoV-2 S-frag4_nostop
Plasmid#153176PurposeGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS-frag4
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pαH-S-GSAS-B.1.351.v6
Plasmid#173791PurposeMammalian expression of SARS-CoV-2 Spike protein South African variant version 6DepositorInsertSpike (S-GSAS-B.1.351 variant version 6) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 SARS-CoV-2 S-frag1_nostop
Plasmid#153173PurposeGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS-frag1
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221 SARS-CoV-2 S-frag6_nostop
Plasmid#153184PurposeGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS-frag6
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJune 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221 SARS-CoV-2 S-frag2_nostop
Plasmid#153174PurposeGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS-frag2
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pαH-S-GSAS-B.1.351.v5
Plasmid#173790PurposeMammalian expression of SARS-CoV-2 Spike protein South African variant version 5DepositorInsertSpike (S-GSAS-B.1.351 variant version 5) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.351.v3
Plasmid#173787PurposeMammalian expression of SARS-CoV-2 Spike protein South African variant version 3DepositorInsertSpike (S-GSAS-B.1.351 variant version 3) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHBS941 TDP43 RRM-GFP VLIMFYW-S
Plasmid#121992PurposeTo test the effect of sequence on TDP43 RRM-GFP droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll hydrophobic residues in IDR1&IDR2 mutated…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only