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Showing: 801 - 850 of 1663 results
  1. pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA

    Plasmid
    #140223
    Purpose
    Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activator
    Depositor
    Insert
    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    PGK
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pF(UG) hSyn NS34a-msGFP-'S-mito

    Plasmid
    #158768
    Purpose
    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Insert
    NS3/4a protease and msGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
  3. pDV06.2c_DART-VADAR_iRFP720-sensor

    Plasmid
    #200410
    Purpose
    pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2
    Depositor
    Insert
    Closed-loop DART VADAR sensor detecting iRFP720
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid
    #200403
    Purpose
    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detection
    Depositor
    Insert
    Closed-loop DART VADAR sensor entry cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid
    #200402
    Purpose
    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection
    Depositor
    Insert
    Closed-loop DART VADAR sensor entry cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. Nrxn3α-AS4-

    Plasmid
    #184332
    Purpose
    Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.
    Depositor
    Insert
    Nrxn3-alpha (Nrxn3 Mouse)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 801 - 850 of 1663 results