-
Plasmid#140223PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activatorDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
UseTagsExpressionMammalianMutationD832APromoterPGKAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4A RNAiR
Plasmid#115172Purposeexpresses RNAi-resistant Sema4A with N-terminal myc tagDepositorInsertSema4A (Sema4a Mouse)
UseTagsmycExpressionMammalianMutationSilent mutation between amino acids in ORF (401-4…PromoterCMVAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCML 313
Plasmid#98586PurposepNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).DepositorInsertcsrB
UseTagsExpressionBacterialMutationPromoterpLacOAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCML 314
Plasmid#98584PurposepCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).DepositorInsertsCsrA
csrB
UseTagsExpressionBacterialMutationPromoterpLacOAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-Mst2 SARAH
Plasmid#84298PurposeExpresses Mst2 SARAH domain for interaction and spectroscopy studies.DepositorInsertMst2 (STK3 Human)
UseTagsGSTExpressionBacterialMutationL443WPromotertacAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Cx43-7
Plasmid#57603PurposeLocalization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertCx43
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET14b-PITPalpha N90F
Plasmid#58257Purposebacterial expression of human PITP alpha N90FDepositorInsertPITP alpha (PITPNA Human)
UseTagsHISExpressionBacterialMutationN90F (abolishes PI binding)PromoterT7Available sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSETC PITPbeta N89F
Plasmid#58279Purposebacterial expression of PITP beta N89F mutantDepositorInsertPITP beta (PITPNB Human)
UseTagsHISExpressionBacterialMutationN89F (abolishes PI binding)PromoterT7Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET14b-PITPalpha K61A
Plasmid#58258Purposebacterial expression of human PITP alpha K61ADepositorInsertPITP alpha (PITPNA Human)
UseTagsHISExpressionBacterialMutationK61A (abolishes PI binding)PromoterT7Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-His3MX6-PGAL1-GST
Plasmid#41613DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-H122Y
Plasmid#33085DepositorInsertYAP (YAP1 Human)
UseTagsFlagExpressionMammalianMutationH122YPromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn NS34a-msGFP-'S-mito
Plasmid#158768PurposeLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseLentiviralTagsExpressionMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) CamKII sf iGluSnFR S72A
Plasmid#179726PurposeLentiviral expression of iGluSnFR biosensor for monitoring synaptic vesicle exocytosis and glutamate releaseDepositorInsertiGluSnFR
UseLentiviralTagsExpressionMutationS72APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha-HaloTag
Plasmid#179715PurposeLentiviral expression of mouse Syt7 with C terminal HaloTagDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-deltaTMD SYT7alpha
Plasmid#179718PurposeLentiviral expression of synaptic vesicle-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTag
Plasmid#179719PurposeLentiviral expression of synaptic vesicle-targeted Halo tagged mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn PM-SYT7alpha deltaTMD-HaloTag
Plasmid#179721PurposeLentiviral expression of plasma membrane-targeted Halo tagged mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD
Plasmid#179722PurposeLentiviral expression of lysosome-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag
Plasmid#179723PurposeLentiviral expression of lysosome-targeted Halo tagged mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) U6-SYT7 sgRNA 777 HaloTag
Plasmid#179724PurposeU6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITIDepositorInsertsgRNA and HaloTag
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1dn
Plasmid#174824PurposeMammalian expression of human MLH1dn (codon optimized)DepositorInserthuman MLH1dn (codon optimized)
UseTagsExpressionMammalianMutationdeletion of residues 754-756PromoterEF1aAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDV06.2c_DART-VADAR_iRFP720-sensor
Plasmid#200410PurposepDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2DepositorInsertClosed-loop DART VADAR sensor detecting iRFP720
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES
Plasmid#200403PurposeEntry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detectionDepositorInsertClosed-loop DART VADAR sensor entry cassette
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP
Plasmid#71237PurposeExpress sgRNA and dCas9-KRAB from 3rd generation lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A GFP
sgRNA
UseCRISPR and LentiviralTagsFlagExpressionMutationD10A and H840APromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES
Plasmid#200402PurposeEntry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detectionDepositorInsertClosed-loop DART VADAR sensor entry cassette
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_18xTEAD_luc2
Plasmid#173003Purposefirefly luciferase reporter vector with 18x clustered TEAD binding sitesDepositorInsert18xTEAD-luc2
UseAAV and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124428PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-flag YAP2 5SA
Plasmid#27371DepositorInsertYAP2 (YAP1 Human)
UseTagsflagExpressionMammalianMutationSerines at positions 61, 109, 127, 128, 131, 163,…PromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-mMLH1dn
Plasmid#174825PurposeMammalian expression of mouse MLH1dn (codon optimized)DepositorInsertmouse MLH1dn (codon optimized)
UseTagsExpressionMammalianMutationdeletion of residues 758-760PromoterEF1aAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-mRxxFP1(AfeI)-iP-A
Plasmid#204625PurposeCloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmax pona 12xTRICK 24xMS2SL
Plasmid#64542PurposeTRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.DepositorInsert12x PP7 stem-loops
UseTags24xMS2 stem loops, HA, NLS, and dNP799ExpressionMammalianMutationPromoterponAAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-HSP70
Plasmid#163790PurposeExpresses mScarlet-Hsp70 in mammalian cellsDepositorInsertHSP70 (HSPA1A Human)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti U6-sgRNA/EF1a-mCherry
Plasmid#114199PurposeLentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoterDepositorInsertsgRNA scaffold
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterU6Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCCL/IFNB1-d2eGFP-3’UTR
Plasmid#180232PurposeReporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1DepositorInsertd2eGFP
UseLentiviralTagsExpressionMutationPromoter1 kb upstream of IFNB1 CDSAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii)
Plasmid#36880DepositorInsertstTA2
tdT-3Myc
insulator
UseTags3 Myc tagsExpressionMammalianMutationinsertion of a beta-globin intron with a loxP sit…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
lenti EF1a-FLAG-dCas9-VPR
Plasmid#114195PurposeLentivirus compatible dCas9-VPR construct driven by the EF1a promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_FLAG-YAP1
Plasmid#173001Purposeconstitutive expression of FLAG-tagged YAP1DepositorInsertFLAG-YAP1 (YAP1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIH-Myc-YAP-5SA
Plasmid#33093DepositorInsertYAP (YAP1 Human)
UseRetroviralTagsMycExpressionMammalianMutationS61A, S109A, S127A, S164A, S381A (phosphorylation…PromoterCMV/MSVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-SynTetOff-GFP
Plasmid#190231PurposeAAV vector, high-level expression of EGFP in neuronal cellsDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-EGFP:LIMD1
Plasmid#108230PurposeLentiviral vector for Tet-inducible LIMD1 expressionDepositorInsertLIMD1 (LIMD1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterTet/ON Minimal CMV promoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
CaTCH BC1
Plasmid#154195PurposeLentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)DepositorInsertCaTCH BC1
UseLentiviral; Catch barcode labelingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nrxn3α-AS4-
Plasmid#184332PurposeExpression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.DepositorInsertNrxn3-alpha (Nrxn3 Mouse)
UseTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Dendra2-Vinculin-N-21
Plasmid#57749PurposeLocalization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573DepositorInsertNM_003373.3 (VCL Human)
UseTagsDendra2ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti6_YAP1-V5
Plasmid#173002Purposeconstitutive expression of V5-tagged YAP1DepositorInsertYAP1-V5 (YAP1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
CaTCH_empty-backbone
Plasmid#157746PurposeLentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequencesDepositorTypeEmpty backboneUseLentiviral; Catch barcode cloningTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1NTD-NLS
Plasmid#174826PurposeMammalian expression of human MLH1NTD-NLS (codon optimized)DepositorInserthuman MLH1_NTD-NLS (codon optimized)
UseTagsSV40 bpNLSExpressionMammalianMutationtruncation of residues 1-335PromoterEF1aAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-3xFLAG-YAP1-S127A
Plasmid#42239DepositorInsertYAP1 (YAP1 Human)
UseGatewayTags3xFLAGExpressionMutationSerine 127 changed to alaninePromoternoneAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 EGFPC2 TAZ
Plasmid#66850PurposeExpress GFP-fused TAZ by lentivirusDepositorInsertWWTR1/TAZ (WWTR1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
T7-Elongin C-pcDNA3
Plasmid#19998DepositorInsertElongin C (ELOC Human)
UseTagsT7-tagExpressionMammalianMutationPromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFl Strep-Sumo-TEV human OAS1
Plasmid#130895PurposeHuman oligoadenylate synthase 1 (OAS1) is used to label terminal ADP-riobosDepositorInsertoligoadenylate synthase 1 (OAS1 Human)
UseTagsStrep_SumoExpressionInsectMutationPromoterphAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only