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Plasmid#66216PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertADRA2A (ADRA2A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKIKOlacZKm
Plasmid#46767PurposeE.coli genomic integration at lacZDepositorTypeEmpty backboneUseE.coli genomic integrationTagsExpressionMutationPromoterAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato-NLS. PGK-puro
Plasmid#136261PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomato-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-yoHaloTag-TLG1
Plasmid#105277PurposePlasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.DepositorInsertTlg1 (TLG1 Budding Yeast)
UseTagsyoHaloTagExpressionYeastMutationHaloTag has yeast optimized codonsPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF0175
Plasmid#141542PurposeLentiviral vector for overexpressing the RELA transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRELA (RELA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TC1614
Plasmid#164873PurposeCURE-X: C to U RNA Editing, with low off-target effects but also lower efficiencyDepositorInserthAPOBEC3A inserted into RfxCas13d (APOBEC3A )
UseCRISPRTagsExpressionMammalianMutationhuman apobec Y132DPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB_iMYOD1_P2A_GFP_Puro
Plasmid#168804PurposePiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expressionDepositorInsertMYOD1 (MYOD1 Synthetic, Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7-L1T1L-NLuc
Plasmid#128942PurposeExpresses L1-NLuc geneDepositorInsertL1 minor core protein
UseTagsNanolucExpressionBacterialMutationPromoterT7 promotorAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGZ108 (pFA6a-3FLAG-MNase-kanMX6)
Plasmid#70231PurposeChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pmIL-6promoterEGFP
Plasmid#112896PurposeTo monitor the activation of the murine IL-6 promoter by eGFP expressionDepositorInsertmurine IL-6 promoter (Il6 Mouse)
UseTagsExpressionMammalianMutationPromoterIL-6 promoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry
Plasmid#170185PurposeExpression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.DepositorInsertsEc86 RT
Retron Ec86 msr-msd
BFP-to-GFP conversion donor template An
UseCRISPRTagsNLSExpressionMammalianMutationPromoterCBh and hU6Available sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Zdk1-EB1C
Plasmid#107696PurposeC-terminal half of π-EB1, EGFP-tagged, dissociates from microtubule ends in blue lightDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsEGFP and Zdk1ExpressionMammalianMutationEB1 aa 186-268PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-lck-smMyc-4x6T-WPRE
Plasmid#196415PurposeAstrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorInsertLck-smMyc
UseAAV; Astrocyte-selectiveTagsLckExpressionMammalianMutationPromoterCAG and GfaABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 p18 shRNA
Plasmid#26631DepositorInsertp18 shRNA (LAMTOR1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
Flag-neo-PP1-Beta
Plasmid#171269PurposeMammalian expression of 3x-Flag-tagged human PP1 beta catalytic subunitDepositorInsert3x-Flag-tagged human PP1 beta catalytic subunit (PPP1CB Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-SpEndoH
Plasmid#218196PurposeBacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoHDepositorInsertEndoH
UseTagstufA-His-CBDExpressionBacterialMutationcodon optimized Ecoli, residues 45-313PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
p323.hSyn.Halo-mFMRP.Wpre
Plasmid#185909PurposeExpresses FMRP fused to HaloTag for protein localization through fluorescence imagingDepositorInsertHaloTag fused to mouse Fragile X mental retardation protein (Ctsa Mouse)
UseLentiviralTagsHaloTagExpressionMutationPromoterhSyn (human Synapsin promoter)Available sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCCL-GKL1RC-Short-LEUg1mKateg2GKL2ORF10UCS
Plasmid#52345PurposeIntegration cassette to create p1-Short-Leu-mKate with p2 ORF10 UCS>mKateDepositorInsertmKate2
UseP1 integration vectorTagsExpressionBacterialMutationchanged V2HPromoterpGKL2 ORF10 UCSAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-A2UCOE-EF1a-iCasp9-P2A-Crry-T2A-mCD59-E2A-Qa1 SCT-F2A-neo-AAVS1
Plasmid#205445Purposehuman AAVS1-targeting plasmid for expression of iCasp9, Crry, mouse CD59, and Qa1 SCTDepositorInsertiCasp9-Crry-mCD59-Qa1 SCT
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only