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pCK302
Plasmid#87768PurposeAs pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be usedDepositorInsertsPrhaBAD-sfGFP
rhaS from E. coli with its native RBS from E. coli
UseSynthetic BiologyTags6xHis TagExpressionBacterialMutationPromoterPrhaBAD rhamnose-inducible promoter from E. coli …Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHL 1915
Plasmid#53022PurposeAmpR + PLlacO1fims::gfp::Asp terminator + CamR + ColE1 (PLlacO1fimS is a fused PLlacO-1 and fimS promoter)DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLlacO-1 and fimS fusionAvailable sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHflu2
Plasmid#203880PurposepHI1 with insertion of ampicillin-resistance geneDepositorInsertbroad-spectrum class A beta-lactamase TEM-116
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet15b _yein
Plasmid#220226PurposeBacterial expression of YeiN in E. coli BL21_DE3 (pLys) or similarDepositorInsertpsug (psuG E.coli)
UseTagsHistagExpressionBacterialMutationPromoterT7Available sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Mortierella verticillate (delta prenylation motif)
Plasmid#170788PurposeExpresses pET-15b(+) Mortierella verticillate (delta prenylation motif)DepositorInsertMortierella verticillate PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lacIAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHL 636
Plasmid#53015PurposeAmpR + PLtetO-1::RBS (st7) fimE::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1DepositorInsertsfimE
fimB
UseSynthetic BiologyTagsAsp terminator, RBS(st7), and T1 terminatorExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Toxoplasma gondii (delta prenylation motif)
Plasmid#170789PurposeExpresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)DepositorInsertToxoplasma gondii PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lacIAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC21
Plasmid#49787PurposeE. coli cloning vector (AmpR, high copy, blue/white selection, M13 IR)DepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCSaE500
Plasmid#53182PurposeInducible expression of E gene which produces a lysis protein that induces autolysis.DepositorInsertpCSaE500
UseTagsExpressionMutationPromoterampR-PampC cassetteAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only
