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Plasmid#75446PurposePlasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pstop
Plasmid#109066Purposeinserts a polyA site to end transcription of a targetted geneDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
6798 pJC25NMstop
Plasmid#1228DepositorInsertNM (SUP35 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pHBS1202 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 4xΦ]
Plasmid#107845PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll hydrophobics combined in four clusters in spl…PromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1203 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 2xΦ]
Plasmid#107844PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll hydrophobics combined in two clusters in spli…PromoterAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLU
Plasmid#64174Purposeharbours marker genes LEU2 and URA3DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046248
Plasmid#153865PurposeYeast expression plasmid for SARS-CoV-2 helicaseDepositorInsertSARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpTEF1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046704
Plasmid#149163PurposeYeast Expression plasmid for SARS-CoV-2 helicaseDepositorInsertSARS-CoV-2 helicase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046213
Plasmid#148986PurposeYeast Expression plasmid for SARS-CoV-2 helicaseDepositorInsertSARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;StrepIIExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceMay 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUK
Plasmid#64185Purposeharbours marker genes URA3 and LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-nlsGFP pA
Plasmid#80814PurposenlsGFP with pADepositorInsertnlsGFPpA 3'E
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD3-proD/tdTomato-hph
Plasmid#120327PurposeBacterial tdTomato labelingDepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterproDAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGLx2-SET1
Plasmid#53256PurposeGAL driven LexA-tagged SET1DepositorInsertSET1
UseTagsLexAExpressionYeastMutationPromoterGAL1/10Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
BB1_P_12_asn_coxA
Plasmid#90290PurposeBB1 promoter coxA gene of Aspergillus niger between FS1 and FS2DepositorInsertcoxA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB1_L_23_syn_BsaI
Plasmid#89915PurposeLinker to construct BB1 from PCR product with FS2 and FS3 (in with BsaI, out with BbsI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHK
Plasmid#64187Purposeharbours marker genes HIS3 and LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAH3
Plasmid#41827PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.DepositorTypeEmpty backboneUsePcr templateTagsExpressionYeastMutationPromoterTEFAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
p35ST
Plasmid#51514PurposepMDC32 with T30 TALEN pair followed by the us:NPTII donor moleculeDepositorInsertsT30 TALEN pair
us:NPTII repair template
UsePlant expression t-dnaTagsNLS and flagExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLUK
Plasmid#64184Purposeharbours marker genes LEU2, URA3 and LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHU
Plasmid#64172Purposeharbours marker genes HIS3 and URA3DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only