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Plasmid#168483PurposeMammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K8,9A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (G2A)-mVenus
Plasmid#168482PurposeMammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
C513-E06: attB5r-pA-polyhedrinp>-attB1r
Plasmid#162948PurposeGateway attB5r/attB1r entry clone containing an AcMNPV polyhedrin promoter with upstream polyA, for use in construction of polycistronic baculovirus expression vectorsDepositorInsertAcMNPV polyhedrin promoter with upstream polyA sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E03: attB4-polyhedrinp>His6-GST-nostop-attB1r
Plasmid#162934PurposeGateway attB4/attB1r entry clone for baculovirus expression of His6-GST tagged proteins using the polyhedrin promoterDepositorInsertHis6-glutathione-S-transferase fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1r
Plasmid#162933PurposeGateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoterDepositorInsertHis6-maltose binding protein fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2 3'UTR GAC
Plasmid#110401PurposeLuminescence-based glutaminase GAC isoform 3'UTR-mediated RNA stability reporterDepositorInsertGLS glutaminase (GLS Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19 KGA HA5'-mKO2-3'
Plasmid#110406PurposeHomology donor for glutaminase KGA isoform knock-in, mKO2DepositorInsertGLS glutaminase (GLS Human)
UseCRISPRTagsExpressionMutationPromoternoneAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
C2 png genomic in Casper
Plasmid#112971PurposeP element vector for transposon insertion of png genomic DNA into Drosophila genomeDepositorInsertpng genomic DNA (png Fly)
UseP insertion vectorTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
C2 M2 png-myc in Casper4
Plasmid#112968PurposeP element vector for transposon insertion of Myc-tagged png into Drosophila genomeDepositorInsertpng-myc (png Fly)
UseP insertion vectorTagsmyc epitopeExpressionMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 gnu
Plasmid#112999PurposeFor protein expression and purification of full-length Drosophila gnuDepositorInsertfull length gnu (gnu Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
hsp70:EB3-mKate2
Plasmid#105965Purposeheat shock inducible transgenesisDepositorInsertEB3 (MAPRE3 Human)
UseTagsmKate2ExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME GFP-DCX
Plasmid#105962Purposeentry clone for gateway cloningDepositorInsertNP_835365.1 (DCX Human)
UseTags6xHis-thrombin cleavage site and EGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_N2A
Plasmid#98677PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2ADepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationN1775A, N1782APromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_del1961-1970
Plasmid#98680PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with del1961-1970DepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationdel1961-1970PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_N2A_S1966C
Plasmid#98682PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A and S1966CDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationN1775A, N1782A, S1966CPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_K5S_S1966C
Plasmid#98683PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S and S1966CDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationK1838S, K1859S, K1866S, K1873S, K1887S, S1966CPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
isoform PKC Epsilon (JCE592)
Plasmid#89776Purposeyeast expression of mouse PKC Epsilon with yeast PKC1 promoter and ADH1 terminatorDepositorInsertPKC Epsilon (Prkce Mouse)
UseTagsExpressionMammalianMutationPromoteryeast PKC1 promoter (-600 to -1)Available sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
isoform PKC Eta (JCE615)
Plasmid#89774Purposeyeast expression of mouse PKC Eta with yeast PKC1 promoter and ADH1 terminatorDepositorInsertPKC Eta (Prkch Mouse)
UseTagsExpressionMammalianMutationPromoteryeast PKC1 promoter (-600 to -1)Available sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWB425
Plasmid#74819PurposeGateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
DVK
Plasmid#66072PurposeMoClo Destination Vector: Empty vector for creating new DVK. Cut with SpeI and insert amplified LacZa (based on pSB1K3)DepositorTypeEmpty backboneUseSynthetic Biology; Destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only