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pTSin EGFP

Plasmid

#127695

Purpose

Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence

Depositor

Bryan Roth, Justin English

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pSRE SSG

Plasmid

#127692

Purpose

Plasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.

Depositor

Bryan Roth, Justin English

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

Sindbis Structural Genome

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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V5-LOV-Turbo1-G3BP1_pTRE-G418

Plasmid

#202015

Purpose

expresses LOV-Turbo1-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-LOV-Turbo1-G3BP1

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

pTRE-Tight

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo1-NIK3x_pTRE-G418

Plasmid

#202017

Purpose

expresses LOV-Turbo1-NIK3x in the mammalian nucleolus under dox inducible promoter, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-LOV-Turbo1-NIK3x

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

pTRE-Tight

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pEY17

Plasmid

#191023

Purpose

flp-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-18 CELE_Y48D7A.2 (flp-18 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-Inpp5e(D477N)

Plasmid

#96809

Purpose

phosphatase-dead control for 5HT6-YFP-Inpp5e, CAAX-null

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-Inpp5e(D477N)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-Scl

Plasmid

#131601

Purpose

expression of mouse ScI in mammalian cells

Depositor

Filipe Pereira, Kateri Moore

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Scl (Tal1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-Inp54p(D281A)

Plasmid

#96813

Purpose

phosphatase-dead control for 5HT6-YFP-Inp54p

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-Inp54p(D281A)

Use

Tags

YFP

Expression

Mammalian

Mutation

D281A inactivates phosphatase activity

Promoter

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-PIPK(D253A)

Plasmid

#96811

Purpose

kinase-dead control for 5HT6-YFP-PIPK

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-PIPK(D253A)

Use

Tags

YFP

Expression

Mammalian

Mutation

D253A inactivates kinase activity of PIPK

Promoter

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-Trib3

Plasmid

#131606

Purpose

expression of mouse Trib3 in mammalian cells

Depositor

Filipe Pereira, Kateri Moore

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Trib3 (Trib3 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(+) eGFP p6

Plasmid

#78582

Purpose

Expresses GFP p6 in mammalian cells

Depositor

Jeremy Luban

Article

Gurer et al J Virol. 2005 Jan;79(2):910-7.

Insert

eGFP p6

Use

Tags

Expression

Mammalian

Mutation

HIV-1 HXB2 p6 with conservative mutations (G78A, …

Promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag-Tpr

Plasmid

#60882

Purpose

Expresses Flag-Tagged nucleoporin Tpr in Mammalian Cells

Depositor

Vinay Nandicoori

Article

Vomastek et al Mol Cell Biol. 2008 Nov;28(22):6954-66.

Insert

Tpr (TPR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

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Human NANOS3-P2A-mCherry knock-in targeting construct

Plasmid

#59721

Purpose

Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

P2A mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-173-N-ORAI1

Plasmid

#87618

Purpose

N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Venus173-N-ORAI1 (ORAI1 Human, Synthetic)

Use

Tags

6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+ EB3-mKate2

Plasmid

#105940

Purpose

in vitro transcription

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

EB3 (MAPRE3 Human)

Use

Tags

mKate2

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-HNRNPAB-3'UTR

Plasmid

#182249

Purpose

Human HNRNPAB 3` UTR region containing 2x binding sites for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of HNRNPAB gene

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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HS-EB3-GFP

Plasmid

#105948

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

EB3 (MAPRE3 Human)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-MYB-3'UTR

Plasmid

#182250

Purpose

Human MYB 3` UTR region containing 3x binding sites for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of MYB gene

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-PDAP1-3'UTR

Plasmid

#182251

Purpose

Human PDAP1 3` UTR region containing 4x binding sites for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PDAP1 gene

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-PIK3R1-3'UTR

Plasmid

#182252

Purpose

Human PIK3R1 3` UTR region containing 1x binding site for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PIK3R1 gene

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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