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Plasmid#212746PurposeDoxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(63)-NLS-VSV
Plasmid#212756PurposeDoxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-CUP1-NUbo-E3(63) I227A-VSV
Plasmid#212757PurposeCopper-inducible expression of NUbo-E3(63) I227A-VSV in budding yeast, reduced activity of E3(63)DepositorInsertE3(63) I227A
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPib1(I227A)PromoterCUP1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-21a-PE2-His
Plasmid#201973PurposeExpress His-tagged PE2 in bacteriaDepositorInsertPE2-HIS
UseTagsHis and c-myc NLSExpressionBacterialMutationPromoterAvailable sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
H2B-mGreenLantern
Plasmid#164464PurposeLocalization of mGreenLantern fluorescent protein to histone 2BDepositorInsertmGreenLantern
UseTagsHuman Histone 2B (D26G and V119I mutations)ExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-hsp70I:QFGal4-SV40pA
Plasmid#155120PurposeTol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInserthsp70l:QFGal4 (hsp70l Zebrafish, Neurospora crassa, Budding Yeast)
UseZebrafish expressionTagsExpressionMutationPromoterhsp70lAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-acta1:QFGal4-SV40pA
Plasmid#155121PurposeTol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertacta1:QFGal4
UseZebrafish expressionTagsExpressionMutationPromoteracta1Available sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pNS19-LV-mutRFP-2A-GFP
Plasmid#113716PurposeLentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)DepositorInsertmutRFP-2A-GFP-IRES-Puro
UseLentiviral; Reporter systemTagsExpressionMammalianMutationY64L (mutRFP)PromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo(K48R)-GFP
Plasmid#212793PurposeCopper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeastDepositorInsertHis6-CUbo(K48R)-GFP
UseIntegrative vectorTagsExpressionYeastMutationCUb(K48R)PromoterCUP1Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo(K63R)-GFP
Plasmid#212794PurposeCopper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeastDepositorInsertHis6-CUbo(K63R)-GFP
UseIntegrative vectorTagsExpressionYeastMutationCUb(K63R)PromoterCUP1Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-E3(48) aa24-150-VSV
Plasmid#212747PurposeConstitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)DepositorInsertE3(48) aa24-150
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationCue1 ΔU7BDPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-ADAM29 (S112F)
Plasmid#31145DepositorInsertADAM29 (ADAM29 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationSerine 112 changed to PheylalninePromoterAvailable sinceSept. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBabe H-Ras
Plasmid#18744DepositorInsertH-Ras (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationG12VPromoterAvailable sinceJuly 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-Sangamo-HIS
Plasmid#40787Purposefor e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsC Terminal 6xHISExpressionBacterialMutation+63 C Terminus,Codon Optimized FokI EndonucleasePromoterT7Available sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQCXP CMV/TO RasV12 (w364-1)
Plasmid#22268DepositorInsertHRAS G12V (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationGlycine 12 to Valine (G12V)PromoterAvailable sinceFeb. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_AAMP:1-434
Plasmid#211192PurposeMammalian expression of full-length human AAMP. N-terminal HiBiT tag.DepositorInsertAAMP:M1-R434 (AAMP Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_WDR62:1-1518
Plasmid#211257PurposeMammalian expression of full-length human WDR62 isoform 4. N-terminal HiBiT tag.DepositorInsertWDR62:M1-H1518 (WDR62 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationisoform 4. L850S natural VAR_031299. Q1305L n…PromoterCMVAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_NLE1:1-485
Plasmid#211141PurposeMammalian expression of full-length human NLE1. N-terminal HiBiT tag.DepositorInsertNLE1:M1-R485 (NLE1 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationP6A natural variant (VAR_060327). R169K natural…PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_CORO1A:1-461
Plasmid#211111PurposeMammalian expression of full-length human CORO1A. N-terminal HiBiT tag.DepositorInsertCORO1A:M1-K461 (CORO1A Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only