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pEF-pIgH-ESCargo(FTV,Crimson)

Plasmid

#140162

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)

Use

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIP CMV VHHG4

Plasmid

#182236

Purpose

Expresses GFP nanobody for stable expression in mammalian cells

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Insert

anti-GFP nanobody

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCI syn iGluSnFR

Plasmid

#106123

Purpose

Indicator for glutamate imaging (synapsin promoter)

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR

Use

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Promoter

synapsin

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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JW617

Plasmid

#64809

Purpose

CUC3 expression in yeast

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC3 (CUC3 Mustard Weed)

Use

Tags

GAL4 DNA binding domain and Myc

Expression

Mutation

Promoter

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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FR_HNF1A

Plasmid

#31104

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

HNF1A (HNF1A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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GAL4-DrBphP

Plasmid

#158986

Purpose

Expresses DrBphP fused with an N-terminal GAL4 DNA binding domain in mammalian cells

Depositor

Liangcai Gu

Article

Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

Insert

GAL4-DrBphP

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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LDB-3-p65

Plasmid

#158984

Purpose

Expresses LDB-3 fused with a C-terminal p65 transcriptional activation domain in mammalian cells

Depositor

Liangcai Gu

Article

Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

Insert

LDB-3-p65

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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mouse Drp1(K38A)

Plasmid

#26049

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Drp1 (Dnm1l Mouse)

Use

Tags

Expression

Mammalian

Mutation

K38A GTPase mutant

Promoter

Available since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pET28a_Pro-IL-36α

Plasmid

#214343

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL36A (IL36A Human)

Use

Tags

His-TEV and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pVAX1/mTyr-Cre

Plasmid

#99249

Purpose

Expresses Cre recombinase under tyrosinase promoter

Depositor

Christian Blank

Article

Deken et al Int J Mol Sci. 2016 Dec 20;17(12). pii:

Inserts

Cre recombinase
mTyr enh/prom

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-CD4-FLAG

Plasmid

#51604

Purpose

expresses CD4 with C-terminal FLAG tag

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

CD4 (CD4 Human)

Use

Tags

FLAG and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-CMV-CC3AI

Plasmid

#78909

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

CC3AI

Use

Lentiviral

Tags

HA and Myc

Expression

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
EGFP in pDBEcR-pIND(SP1)/Bla

Plasmid

#201557

Purpose

Ecdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance.

Depositor

Randy Poon

Article

Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

Insert

DBEcR and EGFP

Use

Unspecified; Sleeping beauty transposon

Tags

DBEcR-myc and FLAG-EGFP

Expression

Mammalian

Mutation

Promoter

EF1a and pIMD(SP1)

Available since

July 13, 2023

Availability

Academic Institutions and Nonprofits only

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p15aC-4D1D-5

Plasmid

#107866

Purpose

Bacterial expression of PIS, PI4K and PI4P5K

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-phosphate 5-kinase
phosphatidylinositol 4-kinase β

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

proD and proD (in operon)

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-MCS-hPOT1_IRES-blast

Plasmid

#166411

Purpose

express hPOT1 in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 (POT1 Human)

Use

Lentiviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

ef-1a

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CVm-PTEN

Plasmid

#168032

Purpose

Expresses eCFP-PTEN(M265-N329)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-PTEN(M265-N329)-mVenus

Use

Tags

Hisx6 and myc

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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PERK 4: PERKKD-pGEX4T-1

Plasmid

#21817

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (537-1114) (Eif2ak3 Mouse)

Use

Tags

GST and Myc (9E10)

Expression

Bacterial

Mutation

PERK Kinase Domain. Amino acids 537-1114.

Promoter

Available since

March 29, 2010

Availability

Academic Institutions and Nonprofits only

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pLentiEKAR2G2

Plasmid

#40178

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Use

Lentiviral

Tags

His-Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV)

Plasmid

#140160

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCS-nlstdTomato

Plasmid

#132985

Purpose

full length of nuclear localized tdTomato in pCS expression vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

tdTomato

Use

Tags

NLS and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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