Addgene

We narrowed to 12,138 results for: EGFP

Showing: 3361 - 3380 of 12138 results

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pIZ-EGFP-MYO10-HMM

Plasmid

#87257

Purpose

Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Use

Tags

Expression

Insect

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

OpIE2

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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TRE-Lepr_Short-IRES-eGFP

Plasmid

#196935

Purpose

Doxycycline inducible expression of short Lepr (no signaling) with eGFP marker

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

Lepr (Lepr Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

short isoform, lack of signaling casacade

Promoter

TRE

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn-PdCO-EGFP-WPRE

Plasmid

#198513

Purpose

Expresses optimized PdCO in frame with EGFP under control of human synapsin1 promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has Service

AAV1 and AAV5

Insert

PdCO

Use

AAV

Tags

EGFP and Rho1D4

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Venus-7aa-ActA-pEGFP-C1

Plasmid

#177405

Purpose

To express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.

Depositor

David Andrews

Article

Osterlund et al Protocol Exchange (2022)

Insert

ActA tail anchor (actA Listeria monocytogenes)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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mEYFP-(L)-mCherry2-(L)-mEGFP-(L)-mApple

Plasmid

#182862

Purpose

Hetero-tetramer expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

mEYFP-mCherry2-mEGFP-mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP/golgin-160/3DE

Plasmid

#180631

Purpose

Expression of caspase-resistant golgin-160

Depositor

Carolyn Machamer

Article

Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

Insert

golgin-160/3DE (GOLGA3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D59E, D139E, D311E

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSyn1-EGFP

Plasmid

#153187

Purpose

Mouse synapsin-1 (mSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-Csy4-Cas9n-EGFP-L2

Plasmid

#141046

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Csy4-2A-Cas9n-2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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LifeAct-EGFP-APEX2 in pCSDEST2

Plasmid

#170523

Purpose

Vector for expression of LifeAct APEX2 in mammalian cells. Parton lab clone FTE

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Cavin4 (Cavin4 Mouse)

Use

Tags

APEX2-P2A-mKate2

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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OCT4-eGFP-PGK-Puro

Plasmid

#31937

Purpose

Oct4 targeting donor vector

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Insert

OCT4 (POU5F1 Human)

Use

Targeting donor

Tags

eGFP

Expression

Mutation

Promoter

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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lenti-25xERSE2-minP-EGFP

Plasmid

#159667

Purpose

EGFP reporter plasmid containing 25 tandem repeats of the ER stress response element-2 (ERSE2)

Depositor

Seiichi Oyadomari

Article

Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

Insert

25xERSE2-minP-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRYc-ABE8e-EGFP

Plasmid

#208340

Purpose

Expresses SpRYc-ABE8e in mammalian cells

Depositor

Pranam Chatterjee

Article

Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

Insert

ecTadA(8e)-nSpRYc

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Sc++ (1-1119), SpRY (1111-1368), D10A

Promoter

CMV

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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FUGW-eGFP-ZEB1 3'UTR

Plasmid

#81018

Purpose

FUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscript

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

Zeb1 3'UTR (UTS2R Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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FUGW-UBC-EGFP-2A-BoNT-B (LC)

Plasmid

#185722

Purpose

Lentiviral expression of GFP and botulinum neurotoxin type B light chain from UbC promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

botulinum neurotoxin type B light chain

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSLfa-PUb-EGFP-3'UTR#5

Plasmid

#176337

Purpose

The synthetic 3'UTR#5 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoter

Depositor

Zach Adelman

Article

Chae et al RNA Biol. 2021 Aug 31:1-9. doi: 10.1080/

Insert

3'UTR#5

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO-EGFP-WPRE-pA

Plasmid

#37085

Purpose

Expresses EGFP in cells lacking Cre (Cre-Off)

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

EGFP

Use

AAV and Cre/Lox; Cre-off

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-HDR-mEGFP-camk2a

Plasmid

#104589

Purpose

AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse camk2a

Depositor

Ryohei Yasuda

Article

Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-EGFP-RIP2

Plasmid

#131201

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 (RIPK2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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p23-puro-EGFP-FBL

Plasmid

#179235

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

FBL (FBL Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1alpha

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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eGFP-TRF1 pWzl-Hygro

Plasmid

#19834

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Oct 19. ():.

Insert

Telomere-Repeat binding Factor 1 (Terf1 Mouse)

Use

Retroviral

Tags

enhanced GFP

Expression

Mutation

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only