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pET-KIX (586-672)

Plasmid

#99336

Purpose

Bacterial expression of mouse CBP KIX domain

Depositor

Peter Wright

Article

Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

Insert

CBP KIX (586-672) (Crebbp Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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LADL Anchor

Plasmid

#127664

Purpose

Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

dCas9-CIBN-2A-Puro

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

Cas9 (D10A,H840A) mutant

Promoter

EF1a

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-mNG

Plasmid

#183465

Purpose

To produce AAV with the P3 promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

IgKL-mNeonGreen

Use

AAV

Tags

IgKL secretory signal

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-SYN_mMaroon-KIX-mMaroon

Plasmid

#137009

Purpose

AAV backbone, mMaroon-KIX acceptor for R-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

KIX domain (Crebbp Rat)

Use

AAV

Tags

mMaroon

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pExpreS2-1-CR-PA-10H-SIII

Plasmid

#175447

Purpose

HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

Depositor

Wyatt Yue

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Use

Tags

BiP Secretion Peptide and Thrombin-Protein A-10Hi…

Expression

Insect

Mutation

Promoter

Fused Actin-HSP70

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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3695 pGEX4T-3 MLL AD mut.E

Plasmid

#8867

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

ILPSDIMDFVL --> ILPADIMDFVL

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
3704 pGEX4T-3 MLL AD L2854A

Plasmid

#8876

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

L2854A: ILPSDIMDFVL --> ILPSDIMDFVA

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pMBS-839

Plasmid

#217995

Purpose

sAP1 protein secretion signal

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Secretion signal peptide derived from the secretory protein secreted acid phosphatase 1 (sAP1)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pPAP_scFv_V50A

Plasmid

#183734

Purpose

Expresses anti-lysozyme scFv antibody with V50A mutation on MFalpha signal

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Anti-lysozyme scFv

Use

Tags

His6-tag

Expression

Yeast

Mutation

V50A mutation in MFalpha signal

Promoter

AOX1 promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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3691 pGEX4T-3 MLL AD mut.A

Plasmid

#8863

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

ILPSDIMDFVL --> ILPSDIMDAAA

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
3692 pGEX4T-3 MLL AD mut.B

Plasmid

#8864

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

ILPSDIMDFVL --> ILPSAAAAFVL

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
3693 pGEX4T-3 MLL AD mut.C

Plasmid

#8865

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

ILPSDIMDFVL --> ILPSNIMNFVL

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
3694 pGEX4T-3 MLL AD mut.D

Plasmid

#8866

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

ILPSDIMDFVL --> AAPSDIMDFVL

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
3696 pGEX4T-3 MLL AD I2844A

Plasmid

#8868

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

I2844A: ILPSDIMDFVL --> ALPSDIMDFVL

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
3697 pGEX4T-3 MLL AD L2845A

Plasmid

#8869

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

L2845A: ILPSDIMDFVL --> IAPSDIMDFVL

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
3698 pGEX4T-3 MLL AD D2848N

Plasmid

#8870

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

D2848N: ILPSDIMDFVL --> ILPSNIMDFVL

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
3699 pGEX4T-3 MLL AD I2849A

Plasmid

#8871

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

I2849A: ILPSDIMDFVL --> ILPSDAMDFVL

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
3700 pGEX4T-3 MLL AD M2850A

Plasmid

#8872

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

M2850A: ILPSDIMDFVL --> ILPSDIADFVL

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
3701 pGEX4T-3 MLL AD D2851N

Plasmid

#8873

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

D2851N: ILPSDIMDFVL --> ILPSDIMNFVL

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
3702 pGEX4T-3 MLL AD F2852Y

Plasmid

#8874

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

F2852Y: ILPSDIMDFVL --> ILPSDIMDYVL

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only