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pCMV-TAPtagC-PrP-SBP_L

Plasmid

#146810

Purpose

Mammalian Expression vector

Depositor

Elisa Izaurralde

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagN-PrP-SBP_L

Plasmid

#146812

Purpose

Mammalian Expression vector

Depositor

Elisa Izaurralde

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagN-HsPatL1_70-383_K

Plasmid

#146725

Purpose

Mammalian Expression of HsPatL1_70-383

Depositor

Elisa Izaurralde

Insert

HsPatL1_70-383 (PATL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagN-HsRCK-mut1_K

Plasmid

#146727

Purpose

Mammalian Expression of HsRck-mut1

Depositor

Elisa Izaurralde

Insert

HsRck-mut1 (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-miniCMV-Nluc

Plasmid

#192657

Purpose

A plasmid encoding miniCMV sgRNA and miniCMV-promoter-Nluc

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Nluc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

U6, miniCMV

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-Mfn1-T564A

Plasmid

#191978

Purpose

To produce mutants mimicking dephosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T564A (Mfn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

T564A

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-Mfn1-T564D

Plasmid

#191979

Purpose

To produce mutants mimicking phosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T564D (Mfn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

T564D

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Dest-MBP-Nsp1

Plasmid

#191483

Purpose

Mammalian expression of MBP-tagged SARS-CoV-2 Nsp1

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

MBP-Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-hCMV1-T-V5-GR-IRES-H2BmCherry-pA

Plasmid

#171502

Purpose

Overexpression of T(Brachyury)-GR

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

T

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMVTRE3G_LYSET-Isoform2-I142V-Flag

Plasmid

#192006

Purpose

Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 I142V mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform2-I142V-Flag (LYSET Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMVTRE3G_LYSET-Isoform2-R45W-Flag

Plasmid

#192005

Purpose

Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 R45W mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform2-R45W (LYSET Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_YH/ GG_delta3291-3298

Plasmid

#187127

Purpose

mammalian cell expression of FLAG-HUWE1 Y355G/H356G, delta3291-3298

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Y355G/H356G, delta3291-3298

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pX601‐mhCMV‐miniABEmax‐N3‐ E53ogRNA

Plasmid

#187064

Purpose

Npu Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

miniABEmax-Npu

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

mhCMV

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-miRFP2-IRES-HO1-N1

Plasmid

#178967

Purpose

Expresses miRFP2 and HO1 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

miRFP2

Use

Tags

HO1 and no

Expression

Mammalian

Mutation

NO

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-miRFP670-eDHFR(69K6)

Plasmid

#178859

Purpose

Mammalian expression of miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2393-Tier1-PhCMV-p2a_fastFT

Plasmid

#169530

Purpose

Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P2A-fused fluorescent timer, fast maturation time

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFOX11-Tier1-PhCMV-Clover

Plasmid

#169508

Purpose

Tier-1 vector encoding PhCMV-driven gren fluorescent protein clover (PhCMV-clover-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven green fluorescent protein clover

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAB300-Tier1-PhCMV-rTetR

Plasmid

#169577

Purpose

Tier-1 vector encoding PhCMV-driven tetracycline-activated rTetR (PhCMV-rTetR-VPR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

reverse tetracycline repressor protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pVH19-Tier1-PhCMV-p300core

Plasmid

#169572

Purpose

Tier-1 vector encoding PhCMV-driven histone acetyltransferase p300 core domain expression (PhCMV-p300core-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P300core (EP300 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2373-Tier1-PhCMV-Gal4

Plasmid

#169581

Purpose

Tier-1 vector encoding PhCMV-driven Gal4 DNA binding domain (PhCMV-Gal4-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

regulatory protein GAL4

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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