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Plasmid#206020PurposeAn intact type LINE-1 vector plasmid possessing the scFv-Fc expression unitDepositorInsertmouse LINE1 vector harboring an scFv-Fc expression unit
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CSNK1G2_IDG-K
Plasmid#214459PurposeGateway destination clone of CSNK1G2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CSNK1G2 (CSNK1G2 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAXY0015
Plasmid#205584PurposeNpuDnaE(C)_HYG(90-341)_RUBYDepositorInsertNpuDnaE(C)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0014
Plasmid#205583PurposeHYG(1-89)-NpuDnaE(N)_GFPuvDepositorInsertNpuDnaE(N)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-hCD59-T2A-GFP
Plasmid#205460Purposelentiviral plasmid for expression of human CD59DepositorInserthCD59 (CD59 Human)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
PH-PLCd1-mNGx1
Plasmid#202751Purposeexpresses fluorescent protein-tagged lipid biosensorDepositorInsertPLCD1v2(1-170):mNG2 (PLCD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-β4 hinge-ear
Plasmid#196692PurposeExpresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteriaDepositorInsertAP-4 β4 hinge-ear (534-739) (AP4B1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-LEPR-COMP5AP-AviTag-9xHis
Plasmid#157548PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertLEPR (LEPR Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMW#2
Plasmid#13349DepositorInsertHIS3 reporter
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBW2176_pCAG-PV1-GAI-L1-FlpO-28C-NLS-BGHpA
Plasmid#108748PurposeExpresses aa28 to C-terminus of Flp recombinase fused to GAI CIDDepositorInsertFlp recombinase fragment aa28 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-HPV11 L1L2
Plasmid#52590PurposeExpresses HPV11 L1 and L2 in mammalian cellsDepositorInsertsHPV11 L1
HPV11 L2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO.3G_X7
Plasmid#171213Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses eGFP for selection. Please see cloning information for suggested restriction sitesDepositorInsertN/A; empty backbone
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Artichoke
Plasmid#73320PurposeArtichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationPromoterSFFV and EF1aAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.773
Plasmid#108527PurposeVanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain.DepositorInsertVanRAM + PVanCC-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-Jaws-KGC-tdTomato-ER2
Plasmid#153535PurposeAAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the Syn promoter.DepositorInsertJaws-KGC-tdTomato-ER2
UseAAVTagsER2, KGC, and tdTomatoExpressionMammalianMutationK200R W214FPromoterSynAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Myc-ASC
Plasmid#73952PurposeExpress c-Myc-tagged human ASC in mammalian cellsDepositorInsertPYD and CARD domain containing, PYCARD (PYCARD Human)
UseTagsc-mycExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.LSL.tdTomato
Plasmid#100048PurposeAAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)DepositorInserttdtomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTet-On-TAP
Plasmid#59017PurposeConstruct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.DepositorTypeEmpty backboneUseToxoplasma expressionTagsCalmodulin Binding Peptide fused with a TEV Cleav…ExpressionMutationPromoterRPS13 with Tet Operators InsertedAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMK265 (RAD21-mAC Hygro)
Plasmid#140539PurposeRAD21 tagging with mAID-CloverDepositorInsertRAD21 (RAD21 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs-gw
Plasmid#18656DepositorInsertGateway cassette A
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-Flag-c-Myc
Plasmid#102625PurposeExpresses c-Myc in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
MscL(G22S)61-cpGFP-ERexp
Plasmid#195311PurposeFluorescent reporter/biosensor for membrane tensionDepositorInsertMscL(G22S)61-cpGFP-ERexp
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-PCNA-19-SV40NLS-4
Plasmid#55320PurposeLocalization: Nucleus, Excitation: 399, Emission: 456DepositorInsertPCNA-19 SV40NLS (PCNA Human)
UseTags3xSV40 NLS and mTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
hSyn1-sypHy-RGECO
Plasmid#84078PurposeCombined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosisDepositorInsertsypHy-RGECO
UseTagsExpressionMammalianMutationPromoterhSyn1Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2
Plasmid#86593PurposeExpresses eGFP-tagged human Akt2(WT)DepositorInsertAkt2 (AKT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-tdTomato-WPRE
Plasmid#51503PurposeCan be used to generate AAV virus that will express tdTomato in the presence of CreDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterpCAGAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNTI647 dCas9-Mxi1 TetR KanMX
Plasmid#139474PurposeIntegrates CRISPRi effector and tet-responsive regulatorDepositorInsertsdCas9-Mxi1
Tet Repressor
UseCRISPRTagsMxi1 and NLSExpressionYeastMutationD10A, H840APromoterpGPM1 and pTEF1Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
RC(WW)-LgBit
Plasmid#208931PurposeRecognition domain for in vitro MAPK-activity sensorDepositorInsertWW domain of PIN1 (PIN1 Human)
UseTagsHISx6, LgBit, and MBPExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF1231
Plasmid#144280PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertAFF3 (AFF3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3505
Plasmid#144981PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKLF12 (KLF12 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GCaMP6s
Plasmid#171156PurposeEncodes GCaMP6sDepositorInsertGCaMP6s
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cas9 TLS2
Plasmid#196978PurposeExpresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.DepositorInsertCas9 with TLS2 tag
UseTagsExpressionPlantMutationPromoterpG10-90Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Alpha-V-Integrin-25
Plasmid#53985PurposeLocalization: Focal Adhesions, Excitation: 487, Emission: 509DepositorInsertAlpha-V Integrin (ITGAV Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAW13.lentiguide.mCherry
Plasmid#104375PurposemCherry vector for cloning in guidesDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV CEPIA4mt
Plasmid#58220PurposeGreen fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)DepositorInsertCEPIA4mt
UseTagsmycExpressionMammalianMutationGCaMP2 N263Y E282V E334D M339L F395W D436EPromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA
Plasmid#164615PurposeExpresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.DepositorInsertRenilla luciferase
UseTags24xMS2v5 (3'UTR) and SunTagExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
MCP-M-MLV-RT
Plasmid#181799PurposeMCP-M-MLV-RT (MS2-N55K-MMLVRT)DepositorInsertMCP-M-MLV-RT
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPaGE Pyl TAG FliC T248TAG
Plasmid#160089PurposeEncodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.DepositorInsertsPyrrolysyl tRNA(cua) (Methanosarcina mazei)
Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
Flagellin
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Threonine 248 to TAG stop-codon.PromoterPseudomonas aeruginosa Leu-tRNA native promoter a…Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-Hylight-T152E
Plasmid#193448PurposeA binding-dead fluorescent biosensor for fructose 1,6-bisphosphate (FBP) for negative control experiments (mammalian expression vector)DepositorInsertBinding-Dead Hylight
UseTagsExpressionMammalianMutationThreonine 67 (ACT) mutated to a Glutamic Acid (GA…PromoterCMVAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGK-HELLO-T2A-Cre LV
Plasmid#202003PurposeGenerates lentiviral vector for autochthonous KP-HELLO tumor initiation by intratracheal administration to KP miceDepositorInsertHELLO-T2A-Cre
UseLentiviralTagsFLAGExpressionMutationPromoterpGKAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen
Plasmid#189976PurposePart 3-4-5 plasmid to introduce mNeonGreen into circRNAsDepositorInsertmNeonGreen
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-p53-shRNA-427
Plasmid#25636DepositorInsertp53-shRNA-427 (TP53 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.GCaMP6f.WPRE.SV40
Plasmid#100836PurposeAAV expression of ultrasensitive protein calcium sensor from CAG promoterDepositorHas ServiceAAV1, AAV2, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCAGAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
CE-8e-SpRY
Plasmid#188913PurposeA-to-G base editorDepositorInsertnSpRYCas9 ecTadA-8e
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mRuby2
Plasmid#99114PurposeAn AAV genome with tet-inducible expression of the fluorescent protein mRuby2DepositorInsertmRuby2
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-BFP
Plasmid#137131PurposeIntersectional viral expression of BFP in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-BFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+hSpCas9
Plasmid#51815PurposeEncode hSpCas9 for in vitro transcription with Sp6DepositorInserthumanized S.pyogenes Cas9
UseCRISPR; Medaka, zebrafishTags3xFLAGExpressionMammalianMutationPromoterSP6Available sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_LaG16_2_synNotch_Gal4VP64
Plasmid#85421PurposeLentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic NotchDepositorInsertLaG16_2 GFP nanobody synNotch Gal4VP64
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPLICS LY-MT Short P2A
Plasmid#213614PurposeDetect the short-range Lysosome-Mitochondria contactDepositorInsertSPLICS LY-MT Short P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
psfTn5
Plasmid#79107Purposebacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutationsDepositorInserttransposase 5
UseTagsHisExpressionBacterialMutationE54K and L372PPromoterAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only