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Plasmid#99372Purpose3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)DepositorInsertdCas9-KRAB-T2A-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENN236_CRISPRcharm_Kv2
Plasmid#220839PurposeCRISPRcharm Kv2 expression in mammalian cellsDepositorInsertCRISPRcharm Kv2
UseCRISPRTagsP2A-TagBFPExpressionMammalianMutationN/APromoterCAGAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)
Plasmid#199605PurposeExpression of cell permeable opAsCas12a in E. Coli.DepositorInsertopCas12a-T8N
UseCRISPRTags6xHis, Twin-strep, SUMOExpressionBacterialMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
T7opt in pCAGGS
Plasmid#65974PurposeHuman codon-optimized T7 polymeraseDepositorInsertT7-opt
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-EGFP BlastR
Plasmid#84383PurposeLentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsEGFPExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
dCas9-VPR_P2A_mCherry
Plasmid#154193PurposeLentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry markerDepositorInsertsp-dCas9-VPR_P2A_mCherry
UseLentiviralTagsExpressionMammalianMutationD10A, H840APromoterEF1asAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENN226_CRISPRcharm
Plasmid#220834PurposeCRISPRcharm expression in mammalian cellsDepositorInsertCRISPRcharm
UseCRISPRTagsP2A-TagBFPExpressionMammalianMutationN/APromoterCAGAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mCherry-CHMP4B
Plasmid#116923Purposeexpresses mCherry-CHMP4B in mammalian cellsDepositorInsertCHMP4B (CHMP4B Human)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-YFP
Plasmid#1816PurposeMammalian expression of Lamp1 fused to YFP. Used for localization to the lysosome.DepositorInsertlysosome associated membrane protein 1 (Lamp1 Rat)
UseTagsYFPExpressionMammalianMutationD50E (not important for function of plasmid)PromoterAvailable sinceFeb. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eNme2-T.1-ABE8e
Plasmid#185668PurposeExpresses eNme2-T.1-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-neNme2-T.2
UseCRISPRTagsExpressionMammalianMutationNme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…PromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGIP
Plasmid#26777PurposeLentiviral vector for stable integration of GFP expression cassette with puromycin selection.DepositorInsertEF1a-GFP-IRES-Puro (gfp )
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
Dual-sgRNA_hU6-mU6
Plasmid#154194PurposeLentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 ASXL1 (p.G646 Wfs *12) 3x FLAG
Plasmid#74245PurposeCancer-associated ASXL1 truncation mutant in mammalian expression vectorDepositorInsertASXL1 (ASXL1 Human)
UseTags3X FLAGExpressionMammalianMutationp.G646 Wfs *12 truncationPromoterCMVAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
RfxCas13d-backbone
Plasmid#165078PurposeExpression of RfxCas13d sgRNAs from a U6 promoterDepositorInsertScaffold gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eNme2-T.2-ABE8e
Plasmid#185669PurposeExpresses eNme2-T.2-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-neNme2-T.2
UseCRISPRTagsExpressionMammalianMutationNme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENN496_miniCRISPRcharm_dSaCas9
Plasmid#220837Purposemini CRISPRcharm expression in mammalian cellsDepositorInsertmini CRISPRcharm
UseCRISPRTagsP2A-TagBFPExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEVA228-pro4IUPi
Plasmid#122018PurposeExpresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.DepositorInsertsUseTagsExpressionBacterialMutationCodon optimized for E. coliPromoterpro4Available sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetO-hNGN2-Neo
Plasmid#99378PurposeExpresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.DepositorInserthNGN2-P2A-Neo (NEUROG2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTetOAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIgkc
Plasmid#127157PurposeExpression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant regionDepositorInsertIgkc (Igkc Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only