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Plasmid#40253DepositorInsertLOV-ipaA L514K L531E
UseTags6xHisExpressionBacterialMutationL514K; L531E; Jα helix region hybridized (AsLOV2 …PromoterT7Available sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
HIV-1 integrase F185H/C280S in pET15b
Plasmid#61672PurposeHIV-1 integrase F185H/C280S in pET-15bDepositorInsertHIV-1 integrase F185H/C280S
UseSynthetic BiologyTagsHisExpressionMutationPromoterT7 promotorAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-E. coli Initiation Factor 3 (IF3)
Plasmid#31340DepositorInsertE. coli Initiation Factor 3
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
FL MALT1 Catalytic Mutant in pET29b-Strep
Plasmid#48969PurposeExpresses Full length catalytic mutant MALT1 in e.coliDepositorInsertMALT1 isoform A (MALT1 Human)
UseTagsHis and StrepExpressionBacterialMutationCatalytic Mutant C464APromoterT7Available sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)
Plasmid#117911PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR44A & H45Q on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP8
Plasmid#135553PurposeExpress mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP8
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-N23PP/L28F/G51PEKN
Plasmid#118585PurposeBacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationN23 mutated to P23, L28 mutated to F28, G51 mutat…PromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXB7 (human) HIS-tag pET
Plasmid#8538DepositorInsertHOXB7 (HOXB7 Human)
UseTagsHis and T7ExpressionBacterialMutationPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
HOXB4 (human) HA-TAT-tag pET
Plasmid#8526DepositorInsertHOXB4 (HOXB4 Human)
UseTagsHAExpressionBacterialMutationreplace T7 and HIS tags of pET with an HA tag;PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a His tag-FRB-Venus C
Plasmid#100980PurposeEncodes half of a protein delivery sensor (His-FRB-Venus C) for expression in and purification from BacteriaDepositorInsertHis-FRB-Venus C
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) HIS-tag pET
Plasmid#8492DepositorInsertHoxA9 (Hoxa9 Mouse)
UseTagsHis and T7ExpressionBacterialMutationBamHI/BalI fusion into amino acid #2 of HOXA9; ma…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a SUMO Nup53xl 162-267
Plasmid#86002Purposebacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53DepositorTypeEmpty backboneUseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOXB1 (human) HIS-tag pET
Plasmid#8520DepositorInsertHOXB1 (HOXB1 Human)
UseTagsHis and T7ExpressionBacterialMutationPCR from cDNA clonePromoterAvailable sinceJuly 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
HIV-1 integrase W235F in pET-15b
Plasmid#61681PurposeHIV-1 integrase W235F in pET-15bDepositorInsertHIV-1 integrase W235F
UseTagsHisExpressionMutationW235FPromoterT7Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)
Plasmid#61939PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coliDepositorInsertsUseTags6x HISExpressionBacterialMutationConstruct ends at residue 156PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)
Plasmid#61938PurposeCo-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coliDepositorUseTags6x HISExpressionBacterialMutationConstruct ends at residue 156, residues 2-38 dele…PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIS-Xyl-Xmod-DocIII
Plasmid#60865PurposeCharacteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120CDepositorInsertsXyl
Xmod-DocIII
UseTags6xHISExpressionBacterialMutationThreonine 120 to Cysteine in Xylanase domainPromoterT7Available sinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET30a-ASXL1-HARE-HTH domain
Plasmid#107164PurposeExpresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coliDepositorInsertASXL1 (Asxl1 Mouse)
UseTagsFlagExpressionBacterialMutationContains amino acids 2-83PromoterT7Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FgßF3-ddFLN4-HIS-ybbr
Plasmid#101743Purposemutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertFgßF3 peptide, ddFLN4 fingerprint (FGB Dictyostelium discoideum, Human)
UseTagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4, added 9F in FgßF3PromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXB3 (human) HIS-tag pET
Plasmid#8524DepositorInsertHOXB3 (HOXB3 Human)
UseTagsHis and T7ExpressionBacterialMutationContains 5 additional amino acids before ATG from…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only