Addgene

Showing: 3341 - 3360 of 12138 results

Results per page:

Sort results by:

pEGFP-C3-PLK4 K41M-3xFLAG

Plasmid

#69838

Purpose

This plasmid encodes kinase dead PLK4 isoform 1 (K41M mutation) with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Use

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

K41M

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP Cep170 (Nigg PID200)

Plasmid

#41150

Depositor

Erich Nigg

Article

Guarguaglini et al Mol Biol Cell. 2005 Mar;16(3):1095-107.

Insert

Cep170 (CEP170 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 human cofilin S3E

Plasmid

#50861

Purpose

expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-f1(-)-GAPDH-IRES-EGFP

Plasmid

#115918

Purpose

donor to insert IRES-EGFP at the GAPDH locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

IRES-EGFP

Use

Donor template

Tags

dTomato

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-25xAARE-minP-EGFP

Plasmid

#159666

Purpose

EGFP reporter plasmid containing 25 tandem repeats of the amino acid response element (AARE)

Depositor

Seiichi Oyadomari

Article

Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

Insert

25xAARE-minP-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBI-p53 R175H/EGFP

Plasmid

#16544

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Insert

p53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

R175H

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Basic-8x-gRNA-eGFP

Plasmid

#60718

Purpose

eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mini-donor - NKX2.5-nEGFP

Plasmid

#209287

Purpose

this mini-donor knockin vector can be used to knock nuclear EGFP into NKX2.5 locus

Depositor

Xiaojun Lian

Article

Haideri et al Stem Cell Reports, 2024

Insert

EGFP-nls

Use

Knockin donor vector

Tags

Expression

Mutation

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBMN mEGFP-NDP52(V136S)

Plasmid

#119682

Purpose

For mammalian expression of LIR mutant NDP52

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

NDP52 (CALCOCO2 Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

V136S

Promoter

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEMS-Tom20-mEGFP-reHaloTagS

Plasmid

#200189

Purpose

Tom20 with reversible HaloTagS variant for fluorescent labeling

Depositor

Jacob Piehler

Article

Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

Insert

Tom20 (TOMM20 Human)

Use

Tags

mEGFP and reHaloTagS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-PDGFRb-IRES2-EGFP

Plasmid

#204354

Purpose

Expresses wild-type PDGFRb gene.

Depositor

Hirofumi Nakatomi

Article

Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

Insert

Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mEGFP-CaMKIIa (T286A)

Plasmid

#127390

Purpose

Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

changed Threonine 286 to Alanine

Promoter

pCAG

Available since

Jan. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
DRH313: FCK-CheRiff-eGFP

Plasmid

#51693

Purpose

Channelrhodopsin with improved sensitivity and minimal red light photocurrent

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

CheRiff-eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBMN mEGFP-OPTN(F178A)

Plasmid

#119681

Purpose

For mammalian expression of LIR mutant OPTN

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

OPTN (OPTN Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

F178A

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pegfp-N1-TFEB-S3A,R4A

Plasmid

#44446

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed both Serine 3 and Arginine 4 to Alanine

Promoter

CMV

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-EGFP-AID-BubR1

Plasmid

#47330

Purpose

for plasmid integration using the Flp-In system. AID at N-terminal

Depositor

Don Cleveland

Article

Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

Insert

BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)

Use

Tags

AID and GFP

Expression

Mammalian

Mutation

siRNA resistant

Promoter

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pdCas9-DNMT3A-EGFP (ANV)

Plasmid

#71685

Purpose

Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA

Depositor

Vlatka Zoldoš

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, S. pyogenes, Human)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9; E756A inactiva…

Promoter

CBh

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-CMV-MCS-Puro-EGFP

Plasmid

#219795

Purpose

Mammalian expression plasmids using piggyBac Transposon System

Depositor

Keiichi Nakayama

Type

Empty backbone

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE-EGFP-FLAG-PLIN3

Plasmid

#161918

Purpose

Expresses human PLIN3 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

Perilipin-3 (PLIN3 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pIZ-EGFP-MYO10-HMM

Plasmid

#87257

Purpose

Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Use

Tags

Expression

Insect

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

OpIE2

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge