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Plasmid#47938Purposeexpresses Myc tagged human MOB1 containing T12A and T35A mutationsDepositorInsertMOB1A (MOB1A Human)
UseTagsMycExpressionMammalianMutationT12A and T35APromoterCMVAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBactin-mNeonGreen-CI
Plasmid#196850PurposeExpression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoterDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationNone (wt)PromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cx43-Myc
Plasmid#60693Purposemammalian expression of Myc-tagged rat Cx43DepositorInsertCx43 (Gja1 Rat)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1Gh
Plasmid#44507DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-EF-5Nanog_2
Plasmid#174871PurposeCRISPR vector for generating Nanog STREAMING-tag KI cellDepositorInsertsgRNA for mouse Nanog (Nanog Synthetic)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPAT-11AA-2XStrep
Plasmid#125680Purposeexpresses human PPAT protein, with first 11 residues removed, with a C-terminal 2XStrep affinity tagDepositorInsertPPAT (PPAT Human)
UseTags2X Strep-Tag IIExpressionMammalianMutationthe first eleven residues have been removedPromoterCMVAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cx43-YA/VD
Plasmid#60699Purposemammalian expression of rat Cx43 YA/VD mutantDepositorInsertCx43 (Gja1 Rat)
UseTagsExpressionMammalianMutationYA/VD (Y286A/V289D)PromoterCMVAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
FCGR2a-bio-His
Plasmid#51754PurposeExpresses full-length Low affinity immunoglobulin gamma Fc region receptor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertFCGR2a (FCGR2A Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
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LXSN-Cx43-YA/VD
Plasmid#60700Purposeretroviral expression of rat Cx43 YA/VD mutantDepositorInsertCx43 (Gja1 Rat)
UseRetroviralTagsExpressionMammalianMutationYA/VD (Y286A/V289D)PromoterLTRAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTK Cas9-2A-Citrine
Plasmid#92393PurposeEnhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.DepositorInsertCas9 2A Citrine
UseCRISPRTagsExpressionMammalianMutationPromoterthymidine kinase promoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKbeta rat S129A, S133A, S137A
Plasmid#32456DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
UseTagsFLAGExpressionMammalianMutationS129A, S133A, S137A (S3V, G96R, and P108L also pr…PromoterSV40Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
TREML1-bio-His
Plasmid#51857PurposeExpresses full-length Trem-like transcript 1 protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTREML1 (TREML1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCK669
Plasmid#192638PurposeExpresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmRDepositorInsertsSp.pCas9-dxCas9-NG
BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPR and Synthetic BiologyTagsExpressionMutationSoxS has R93A and S101A mutations and dxCas9-NG h…PromoterBBa_J23107 and Sp.pCas9Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT-flk1-cytoBirA-2A-mCherry_Ras
Plasmid#80056PurposeFlk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterflk1Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
TMED2-bio-His
Plasmid#51853PurposeExpresses full-length Transmembrane emp24 domain-containing protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTMED2 (TMED2 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only