-
Plasmid#196704PurposeExpresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cellsDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
UseTagsmCherry and mEGFPExpressionMammalianMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-APPP1-mGFP
Plasmid#196705PurposeAs mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activityDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
UseTagsmCherry and mEGFPExpressionMammalianMutationLys to Val substitution at position 612PromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-P2A-hMLH1dn
Plasmid#222997PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)DepositorInsertPE7-P2A-hMLH1dn
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDeletion of residues 754-756 in MLH1 as describedā¦PromoterCMVAvailable sinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
Plasmid#179106PurposepCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINTDepositorInsertpCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)
Plasmid#179116PurposeCargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)DepositorInsertCargo EGFP with AttP Bxb1 site for in-frame fusion
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)
Plasmid#179115PurposeCargo EGFP with AttP Bxb1 site (Parent minicircle)DepositorInsertCargo EGFP with AttP Bxb1 site
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2
Plasmid#112668PurposeDonor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression of your protein of interest.DepositorInserttdT
UseAAV; Donor plasmid for recombinase-mediated casseā¦TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-BsdCas9-W
Plasmid#67978PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termiā¦ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W
Plasmid#67982PurposeCas9 activity reporter with mCherry and GFPDepositorInsertU6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W
Plasmid#67981PurposeCas9 activity reporter (control) with mCherry and GFPDepositorInsertU6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
Plasmid#67979PurposeCas9 activity reporter (control) with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W
Plasmid#67974PurposeCRISPR gRNA expression vector with an improved scaffold and puro/BFP markersDepositorInsertU6gRNA cassette, PGKpuro2ABFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-Cas9Bsd-W
Plasmid#68343PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termiā¦ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W
Plasmid#67986PurposeCas9 activity reporter with mCherry and BFPDepositorInsertsU6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
mBFP-APP-mGFP
Plasmid#196694PurposeExpresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cellsDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
UseTagsmEGFP and mtagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W
Plasmid#72666PurposeLentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)DepositorInserth7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AP-APP
Plasmid#196706PurposeExpresses APP695 with a biotin Acceptor Peptide tag at N-terminal for single molecule tracking and other measusers in mammalian cellsDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
UseTagsAP, Avitag and HAExpressionMammalianMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W
Plasmid#67985PurposeCas9 activity reporter (control) with mCherry and BFPDepositorInsertU6gRNA cassette, PGKmCherryABFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX313-TP53-WT
Plasmid#118014PurposeConstitutive expression of wild-type human Tumor Protein p53 (TP53)DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HDRT_TRAC_CD19-CAR
Plasmid#183473PurposeEncodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC geneDepositorUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only