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pCK-Drosha(UniProt)-FLAG-mEGFP

Plasmid

#164691

Purpose

Express Drosha-FLAG-mEGFP in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

Drosha (DROSHA Human)

Use

Tags

FLAG and mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLL5.0 shCAV1-NLS-mCherry

Plasmid

#187257

Purpose

Lentiviral expression of shRNA targeting Caveolin1 and NLS-mCherry

Depositor

Alpha Yap

Article

Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

Insert

ShRNA for Caveolin-1 (Cav1 Mouse)

Use

Lentiviral

Tags

NLS-mCherry

Expression

Mutation

Promoter

U6

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mCherry-CyrCR1.mutant

Plasmid

#128746

Purpose

Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)

Use

Tags

Expression

Mammalian

Mutation

5 point mutations disrupting the miR-7 site

Promoter

CMV

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-S32

Plasmid

#140308

Purpose

Expresses HA-tagged human Src-S32 in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

contains only the SH3 and SH2 domains

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCK-D390TN(UniProt)-FLAG

Plasmid

#164693

Purpose

Express N-terminally truncated DroshaTN-FLAG in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

N-terminally truncated transdominant negative Drosha (DROSHA Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir451-CMV2-mir1

Plasmid

#164635

Purpose

Express miR-451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR451a (MIR451A Human)
miR-1-1 (MIR1-1 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP

Plasmid

#103817

Purpose

Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP (CRY2 Mustard Weed)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-iRFP713

Plasmid

#103818

Purpose

Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-iRFP713 (CRY2 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCK-D390(UniProt)-FLAG

Plasmid

#164692

Purpose

Express N-terminally truncated Drosha-FLAG in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

N-terminally truncated Drosha (DROSHA Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144m5~451-CMV2-mir1

Plasmid

#164679

Purpose

Express miR-144(mutant5)~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144(mutant5)~451 (MIR144 Human)
miR-1-1 (MIR1-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144m4~451-CMV2-mir1

Plasmid

#164678

Purpose

Express miR-144(mutant4)~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144(mutant4)~451 (MIR144 Human)
miR-1-1 (MIR1-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144m3~451-CMV2-mir1

Plasmid

#164677

Purpose

Express miR-144(mutant3)~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144(mutant3)~451 (MIR144 Human)
mir-1-1 (MIR1-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144m2~451-CMV2-mir1

Plasmid

#164676

Purpose

Express miR-144(mutant2)~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144(mutant2)~451 (MIR144 Human)
miR-1-1 (MIR1-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBI-CMV1-mir144m1~451-CMV2-mir1

Plasmid

#164675

Purpose

Express miR-144(mutant1)~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144(mutant1)~451 (MIR144 Human)
miR-1-1 (MIR1-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir125~451-CMV2-mir1

Plasmid

#164669

Purpose

Express miR-125a~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-125a~451 (MIR125A Human)
miR-1-1 (MIR1-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST40-2XFL-Src-S32

Plasmid

#140309

Purpose

Expresses Flag-tagged human Src-S32 in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Use

Tags

2X Flag

Expression

Mammalian

Mutation

contains only the SH3 and SH2 domains

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-mir451 EF1Alpha-puro-T2A-BFP

Plasmid

#164792

Purpose

Express miR-451 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

microRNA 451a (MIR451A Human)
puro-T2A-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha and U6

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV_IRES_GFP_CD16a

Plasmid

#196189

Purpose

Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

Depositor

Ana Ruiz-Saenz

Article

Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

Insert

CD16a (FCGR3A Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed Phenylalanine 158 to Valine

Promoter

LTR

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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tGFP

Plasmid

#26864

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

Truncated GFP-IRES-Puro

Use

Tags

Expression

Bacterial

Mutation

Deletion of nt 1-36

Promoter

Available since

Nov. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pQCXIP-GFP1-10

Plasmid

#68715

Purpose

Split GFP assay

Depositor

Yutaka Hata

Article

Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

Insert

GFP1-10

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2016

Availability

Academic Institutions and Nonprofits only

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