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pDONR223_VHL_WT
Plasmid#81874PurposeGateway Donor vector containing VHL , part of the Target Accelerator Plasmid Collection.DepositorInsertVHL (VHL Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PDGFRb-IRES2-EGFP
Plasmid#204354PurposeExpresses wild-type PDGFRb gene.DepositorInsertPlatelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
UseTagsExpressionMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCM18
Plasmid#78565Purpose60-copy, promoterless, oriE1(ColE1) luxCDABE, aph, hok-sok, par, parA, rrnB Stabilized luciferase-expressing plasmidDepositorTypeEmpty backboneUseTagsluxCDABEExpressionBacterialMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV GATA2
Plasmid#83820PurposeMammalian expression of mouse Gata2DepositorInsertGATA2 (Gata2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-H-Ras_wt
Plasmid#39503DepositorInsertHRAS (HRAS Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR EGFR-CD3ε-FusionRed
Plasmid#188626PurposeHuman EGFR labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensorDepositorInsertEGFR (EGFR Human, Synthetic)
UseLentiviralTagsCD3ε-FusionRedExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLB052 - deAct - SpVB
Plasmid#136286Purposeentry vector for GreenGate cloning method, D module, open reading frame of the mono(ADP-ribosyl) transferase domain of the Salmonella enterica SpvBDepositorInsertdeAct - SpvB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-132
Plasmid#118518PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLIB-HIS-TEV-Cyclin-B
Plasmid#177012PurposeGenerate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEVDepositorInsertCyclin B1 (CCNB1 Human)
UseTagsPolyhistidine-TEVExpressionInsectMutationcodon-optimised for insect cell expressionPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro FLAG SREBP1
Plasmid#32017DepositorInsertSREBP1 (Srebf1 Mouse)
UseLentiviralTagsFLAGExpressionMammalianMutationV225MPromoterCMVAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
TFORF0267
Plasmid#142502PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
HRE-dUnOHR
Plasmid#201714PurposeHypoxia responsive promoter driving UnaG and mOrangeDepositorInsertUnaG
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
HBA1 full 3'UTR-T4 td downstream intron
Plasmid#189974PurposePart 6 plasmid to introduce the HBA1 full 3'UTR and T4 td downstream intron into circRNAsDepositorInsertHBA1 full 3'UTR-T4 td downstream intron
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ERKKTRClover
Plasmid#59138PurposeORF encoding ERK KTR mClover flanked by Gateway sequencesDepositorInsertERK Kinase Translocation Reporter (MAPK1 Human, Mouse)
UseTagsExpressionMutationPromoterAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
Integrin beta 2 - mYFP
Plasmid#8638DepositorInsertIntegrin beta 2 (ITGB2 Human)
UseTagsYFPExpressionMammalianMutationstop codon of beta2 was replaced with AgeI restri…PromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
TFORF3427
Plasmid#144903PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFIB (NFIB Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
p426-SNR52p-gRNA.CAN1.Y-SUP4t
Plasmid#43803PurposeEncodes a gRNA that targets Can1.Y in yeast.DepositorArticleInsertCAN1.y gRNA (CAN1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3basic luciferase and renilla_polyA
Plasmid#128046PurposepGL3 basic luciferase reporter with co-expression of renilla luciferaseDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-nuclear-EKAR4
Plasmid#174439PurposeNuclear-targeted EKAR4.DepositorInsertnuclear-EKAR4
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX302 MX1-V5 puro
Plasmid#158640PurposeLentiviral expression vector for constitutive expression of V5-tagged MX1.DepositorInsertMx1 (MX1 Human)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-mCD59-T2A-GFP
Plasmid#205451Purposelentiviral plasmid for expression of mouse CD59DepositorInsertmCD59 (Cd59a Mouse)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GST TEV LIC cloning vector (1G)
Plasmid#29655DepositorTypeEmpty backboneUseTagsHis6-GST-TEVExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657
Plasmid#212963PurposeLentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.DepositorInsertDR1_CasRx_GFP targeting spacer
UseCRISPR and LentiviralTagsTagRFP657ExpressionMammalianMutationPromoterU6Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tet-off TauRD (P301L/V337M)
Plasmid#188572PurposeDox-repressible expression of the Tau repeat domainDepositorInsertTau repeat domain (MAPT Human)
UseLentiviralTagsMycExpressionMammalianMutationP301L and V337MPromoterpTight TREAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7
Plasmid#175529PurposeExpression of HaloTag7 at the inner membrane of mitochondria in mammalian cellsDepositorInsertCox8a-HaloTag7
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Lysosomes-20
Plasmid#55308PurposeLocalization: Lysosome Membrane, Excitation: 399, Emission: 456DepositorInsertLAMP1
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFIP
Plasmid#17565DepositorInsertinversion switch
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSANG14-3F
Plasmid#39265DepositorTypeEmpty backboneUseTags6xHis, Alkaline phosphatase, pelB leader, and tri…ExpressionBacterialMutationPromoterT7Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
GreenT-EC - Display - CyRFP1
Plasmid#193943PurposeMammalian expression vector for a low affinity calcium indicator exposed to the cell surfaceDepositorInsertGreenT-EC
UseTagsIgk_secretion - HA-Flag and PDGFRExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
XBP-1 2: pFLAG.XBP1p.CMV2
Plasmid#21833DepositorInsertXBP-1 processed/spliced (Xbp1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
SPELL-TEV Component 2
Plasmid#170490PurposeTEV (C-lobe) fused with FRBDepositorInsertmyc, FRB, TEV(C-lobe)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPELL-TEV Component 1
Plasmid#170489PurposeTEV (N-lobe) fused with iFKBPDepositorInsertTEV(N-lobe), iFKBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
RelA cFlag pcDNA3
Plasmid#20012DepositorInsertRelA (Rela Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
Dead-Laconic/pcDNA3.1(-)
Plasmid#118627PurposeTo express in mammalian cells a lactate FRET sensor negative control.DepositorInsertDead-Laconic
UseTagsExpressionMammalianMutationChange Histidine 151 to Aspartic Acid of Escheric…PromoterCMV PromoterAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-Traditional CAR (anti-CD19)
Plasmid#192115PurposeLentiviral delivery of Traditional CAR(anti-CD19)DepositorInsertpHR-pEF1a-Traditional CAR (anti-CD19scFV_CD28_41BB_CD3z)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLH02
Plasmid#117262PurposeGenome editing for Lactobacillus brevis ATCC367DepositorInsertPorfX-LVISKB_1732-30 , sapK-sappR
UseOtherTagsExpressionMutationPromoterAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXC1
Plasmid#153114PurposeExpression of Flag tagged human FOXC1 in mammalian cellsDepositorInserthuman FOXC1 (FOXC1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC152-dual-dCas9VP64-sgExpression
Plasmid#48238PurposeDual expression construct expressing both dCas9VP64 and sgRNA from separate promotersDepositorInsertdCas9
UseCRISPRTagsHA Tag, HA-Tag, and VP64ExpressionMammalianMutationD10A;H840APromoterAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF0429
Plasmid#143213PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPBX1 (PBX1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57-RFP-P2A-MCS
Plasmid#78933PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero11
Plasmid#187928PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero11DepositorInsertHero11 (C19orf53 Human)
UseTagsFlag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PARP1-Flag-His-WT
Plasmid#111572PurposeExpresses full length PARP1 in Sf9 (insect) cellsDepositorInsertPARP1 (PARP1 Human)
UseTagsC-terminal 6xHis and C-terminal FlagExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Integrin alpha L - mCFP
Plasmid#8637DepositorInsertIntegrin, alpha L (ITGAL Human)
UseTagsCFPExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
ESCRT-II
Plasmid#17633DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 flag p53
Plasmid#10838DepositorInsertp53 (TP53 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
TFORF1683
Plasmid#142826PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTWIST1 (TWIST1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mito-TurboID
Plasmid#200960PurposeTransiently expresses mitochondria localized TurboID with V5 tagsDepositorInsertmito-TurboID
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-eNpHR 3.0-EYFP
Plasmid#26971PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by CaMKIIa promoter for optogenetic inhibitionDepositorHas ServiceAAV1 and AAV9InserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationTrafficking Signal (TS) ER Export SignalPromoterCaMKIIaAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro
Plasmid#83306PurposeLentivirus vector to express guideRNA and dCas9 with puro resistant geneDepositorInsertsgRNA and dCas9 from pX330
UseLentiviralTagsFLAG and PA tagsExpressionMammalianMutationPromoterU6 for sgRNA and CBh for dCas9Available sinceDec. 13, 2016AvailabilityAcademic Institutions and Nonprofits only
