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Plasmid#128835PurposeKnock-in of TagBFP into mouse Esrrb locusDepositorInserttagBFP into Esrrb locus (Esrrb Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMG035 MBP-Axin∆BCD-His
Plasmid#154053PurposeExpresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coliDepositorInsertAxin1 (AXIN1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flag-Avi-ires-CHD5
Plasmid#68878PurposeTransient mammalian expression of CHD5 for BirA taggingDepositorInsertCHD5 (CHD5 Human)
UseTagsFLAG/AVIExpressionMammalianMutationPromoterCAGAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330-Esrrb gRNA
Plasmid#128841PurposegRNA for targeting mouse Esrrb locus using CRISPR-cas techniqueDepositorInsertEsrrb gRNA (Esrrb Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-CHD5(Chromos)-FRAG
Plasmid#68875PurposeIPTG inducible bacterial expression of CHD5 fragmentDepositorInsertCHD5 (CHD5 Human)
UseTagsGSTExpressionBacterialMutationonly the two chromodomains of CHD5PromotertacAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTwist-FLAG-SRSF3_Truncated_Codon.Optimized
Plasmid#172346PurposeExpression of FLAG-tagged, codon-optimized, truncated SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseTagsFLAGExpressionMammalianMutationcodon-optimized, truncated at amino acid 114 with…PromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 - myc ULK1 k46I
Plasmid#27624DepositorInsertmouse myc ULK1 K46I (Myc Mouse)
UseGateway donr vectorTagsmycExpressionMutationInitial M from ULK1 removed. Silent mutation at A…PromoterAvailable sinceFeb. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 - myc ULK1 4SA
Plasmid#27625DepositorInsertmouse myc ULK1 4SA (Myc Mouse)
UseGateway donr vectorTagsmycExpressionMutationS467A, S555A, T574A, S637A. Initial M from ULK1 r…PromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-CHD5-WT-331-672aa
Plasmid#68870PurposeMammalian expression of CHD5 histone binding domain (331-672aa)DepositorInsertCHD5 (CHD5 Human)
UseTagsFLAGExpressionMammalianMutation331-672aaPromoterCMVAvailable sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDV18 [punc-47::SNG-1::CRY2olig(535)]
Plasmid#197598PurposeExpression of SNG-1::CRY2olig(535) in GABAergic motor neurons of C. elegansDepositorUseTagsExpressionWormMutationE490GPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV12 [psng-1::SNG-1::CRY2olig(535)]
Plasmid#197596PurposePan-neuronal expression of SNG-1::CRY2olig(535) in neurons of C. elegansDepositorUseTagsExpressionWormMutationE490GPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH
Plasmid#131684PurposeAn adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1DepositorInsertsEGFP-KASH
SpCas9 sgRNA vs mouse GRID1
UseAAVTagsKASHExpressionMutationPromoterEF1a and mU6-LSL (Cre dependent)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-K142R
Plasmid#213423PurposeExpresses human SKP1 K142R mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-K142R (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-T131A
Plasmid#213482PurposeExpresses human SKP1 T131A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-T131A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-97-101-5A
Plasmid#213642PurposeExpresses human SKP1-97-101-5A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1--97-101-5A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-K163R
Plasmid#213424PurposeExpresses human SKP1 K163R mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-K163R (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-T131E
Plasmid#213601PurposeExpresses human SKP1 T131E mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-T131E (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV06 [punc-17::SNG-1::CRY2(535)]
Plasmid#197597PurposeExpression of SNG-1::CRY2olig(535) in cholinergic motor neurons of C. elegansDepositorUseTagsExpressionWormMutationE490GPromoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(E1418K/A1419R/S1421R/P1424Q/Y1426S)
Plasmid#155010PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationE1418K/A1419R/S1421/P1424Q/Y1426S; insert codes f…PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EphrinB3-Flag(A227Y)
Plasmid#155013PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1DepositorInsertEphrinB3 (EPHB3 Human)
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationA227YPromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only