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pNTKV- Esrrb-KI-3'UTR-2A-TagBFP-RBGpA

Plasmid

#128835

Purpose

Knock-in of TagBFP into mouse Esrrb locus

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

tagBFP into Esrrb locus (Esrrb Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMG035 MBP-Axin∆BCD-His

Plasmid

#154053

Purpose

Expresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

Axin1 (AXIN1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Flag-Avi-ires-CHD5

Plasmid

#68878

Purpose

Transient mammalian expression of CHD5 for BirA tagging

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

FLAG/AVI

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pX330-Esrrb gRNA

Plasmid

#128841

Purpose

gRNA for targeting mouse Esrrb locus using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Esrrb gRNA (Esrrb Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-CHD5(Chromos)-FRAG

Plasmid

#68875

Purpose

IPTG inducible bacterial expression of CHD5 fragment

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

only the two chromodomains of CHD5

Promoter

tac

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pTwist-FLAG-SRSF3_Truncated_Codon.Optimized

Plasmid

#172346

Purpose

Expression of FLAG-tagged, codon-optimized, truncated SRSF3

Depositor

Sujatha Jagannathan

Article

Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

Insert

serine and arginine rich splicing factor 3 (SRSF3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized, truncated at amino acid 114 with…

Promoter

EF-1a

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 - myc ULK1 k46I

Plasmid

#27624

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse myc ULK1 K46I (Myc Mouse)

Use

Gateway donr vector

Tags

myc

Expression

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Promoter

Available since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221 - myc ULK1 4SA

Plasmid

#27625

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse myc ULK1 4SA (Myc Mouse)

Use

Gateway donr vector

Tags

myc

Expression

Mutation

S467A, S555A, T574A, S637A. Initial M from ULK1 r…

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-FLAG-CHD5-WT-331-672aa

Plasmid

#68870

Purpose

Mammalian expression of CHD5 histone binding domain (331-672aa)

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

331-672aa

Promoter

CMV

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
pDV18 [punc-47::SNG-1::CRY2olig(535)]

Plasmid

#197598

Purpose

Expression of SNG-1::CRY2olig(535) in GABAergic motor neurons of C. elegans

Depositor

Alexander Gottschalk

Article

Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

Insert

Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Mustard Weed, Nematode)

Use

Tags

Expression

Worm

Mutation

E490G

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDV12 [psng-1::SNG-1::CRY2olig(535)]

Plasmid

#197596

Purpose

Pan-neuronal expression of SNG-1::CRY2olig(535) in neurons of C. elegans

Depositor

Alexander Gottschalk

Article

Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

Insert

Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Mustard Weed, Nematode)

Use

Tags

Expression

Worm

Mutation

E490G

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH

Plasmid

#131684

Purpose

An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1

Depositor

Christopher Richie

Article

Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

Inserts

EGFP-KASH
SpCas9 sgRNA vs mouse GRID1

Use

AAV

Tags

KASH

Expression

Mutation

Promoter

EF1a and mU6-LSL (Cre dependent)

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-K142R

Plasmid

#213423

Purpose

Expresses human SKP1 K142R mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-K142R (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-T131A

Plasmid

#213482

Purpose

Expresses human SKP1 T131A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-T131A (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-97-101-5A

Plasmid

#213642

Purpose

Expresses human SKP1-97-101-5A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1--97-101-5A (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-K163R

Plasmid

#213424

Purpose

Expresses human SKP1 K163R mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-K163R (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-T131E

Plasmid

#213601

Purpose

Expresses human SKP1 T131E mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-T131E (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDV06 [punc-17::SNG-1::CRY2(535)]

Plasmid

#197597

Purpose

Expression of SNG-1::CRY2olig(535) in cholinergic motor neurons of C. elegans

Depositor

Alexander Gottschalk

Article

Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

Insert

Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Mustard Weed, Nematode)

Use

Tags

Expression

Worm

Mutation

E490G

Promoter

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-EBA175-Flag(E1418K/A1419R/S1421R/P1424Q/Y1426S)

Plasmid

#155010

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfEBA175

Use

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Mutation

E1418K/A1419R/S1421/P1424Q/Y1426S; insert codes f…

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-EphrinB3-Flag(A227Y)

Plasmid

#155013

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

EphrinB3 (EPHB3 Human)

Use

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Mutation

A227Y

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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