We narrowed to 7,540 results for: 11

1. MTK234_033

   Plasmid

   #123996

   Purpose

   Encodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA2 -hAAVS1
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK234_034

   Plasmid

   #123997

   Purpose

   Encodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA3 -hAAVS1
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSIN-Ski-gRNA2

   Plasmid

   #180369

   Purpose

   targeting mouse Ski gene

   Depositor

   Dan Littman

   Article

   Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

   Insert

   Ski targeting gRNA (Ski Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. TALEN-CCR5L18-CtermQ7

   Plasmid

   #51442

   Purpose

   Expresses TALEN targeting left CCR5A site (L18) with Q7 C-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Left (L18) Q7 C-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 EL variant

   Expression

   Mammalian

   Mutation

   K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q

   Promoter

   CMV

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. ATF4 7: 5’3’ATF4.GFP

   Plasmid

   #21854

   Depositor

   David Ron

   Article

   Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

   Insert

   ATF4 (Atf4 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Vector derived viral termination and poly-adenyla…

   Available Since

   Sept. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-GP-2-MPHOSPH8 Chromo

   Plasmid

   #59694

   Purpose

   Contains histone modification interacting domain (MPHOSPH8 Chromo)

   Depositor

   Albert Jeltsch

   Article

   Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

   Insert

   MPHOSPH8 Chromo (MPHOSPH8 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   Sept. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPuroR-MCS-LexA-VP16-MiniWhite

   Plasmid

   #165898

   Purpose

   Puromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable Empty Enhancer Driver
   

   Use

   Synthetic Biology

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-HA-REDD1 48-232 (#182)

   Plasmid

   #99920

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N-terminal deletion; encodes AA 48-232

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. PML-ER-pBabe

   Plasmid

   #73556

   Purpose

   vector encoding a fusion protein between PML-IV and ER (estrogen receptor)

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   PML-IV (PML Human)
   

   Use

   Retroviral

   Tags

   ER

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI

    Plasmid

    #140202

    Purpose

    CRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology; Proof-of-concept te…

    Mutation

    G174D

    Promoter

    PelcA

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbB8k-csg-NbMS2

    Plasmid

    #171701

    Purpose

    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the coat protein of bacteriophage MS2 via a flexible linker.

    Depositor

    Neel Joshi

    Article

    Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1

    Insert

    csgBACEFG, with nanobody fused to CsgA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. VQR variant

    Plasmid

    #138560

    Purpose

    Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VQR
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PDF1::mCitrine-MBD

    Plasmid

    #115222

    Purpose

    Microtubule visualization

    Depositor

    Jan Traas

    Article

    Armezzani et al Development. 2018 Jun 4;145(11). pii: de

    Insert

    Microtubule binding domain
    

    Tags

    mCitrine

    Expression

    Plant

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. TALEN-CCR5R18-CtermQ7

    Plasmid

    #51443

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) Q7 C-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV5 Flag-Smad1 (4SP/AP)

    Plasmid

    #14043

    Depositor

    Joan Massague

    Article

    Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

    Insert

    Smad1 (4SP/AP) (SMAD1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Four Serine to Alanine mutations in the linker re…

    Available Since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-HA-REDD1 77-232 (#426)

    Plasmid

    #99921

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal deletion; encodes AA 77-232

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. MTK4a_020

    Plasmid

    #123833

    Purpose

    Encodes the d4 PEST tag as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    d4PEST tag
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MTK5_012

    Plasmid

    #123856

    Purpose

    Encodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector RE'-A1 Insulator
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. ATF4 10: 5’ATF4.uORF1.GFP

    Plasmid

    #21857

    Depositor

    David Ron

    Article

    Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

    Insert

    ATF4 (Atf4 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    upstream ORF1 (uORF) of ATF4 fused to EGFP

    Available Since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. GB1-PRD(703-800)-Strep

    Plasmid

    #141344

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

    Insert

    GB1-His6-TEV-ALIX(703-800)-Strep
    

    Tags

    GB1-His6-TEV and Strep-tag (WSHPQFEK)

    Expression

    Bacterial

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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