-
Plasmid#167581PurposeExpress the ATP sensor GRAB_ATP1.0 in Cre positive cellsDepositorInsertATP sensor GRAB_ATP1.0 (P2RY1 Human)
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB-Hygro-rKIF5B-GFP
Plasmid#210335PurposeOverexpression of rKIF5BDepositorInsertrKIF5B (Kif5b Rat)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_ATP1.0mut
Plasmid#167580PurposeExpress the ATP-insensitive control sensor GRAB_ATP1.0mut in astrocytesDepositorInsertATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
UseAAVTagsExpressionMutationPromoterGfaABC1DAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9+sgRNA expression vector precursor
Plasmid#171798PurposePICASSO: Library expression vector precursor for paired dCas9+sgRNA expressionDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterpLtetOAvailable sinceAug. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBECAb-apr
Plasmid#122001PurposeA cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumanniiDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR59.4
Plasmid#63175PurposeExpresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgCtr- LentiCRISPRv2
Plasmid#107402PurposeLentiviral expression of Cas9 and a control gRNADepositorInsertcontrol gRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
HP138-neo
Plasmid#134247PurposeCas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selectionDepositorInsertContains Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_ATP1.0mut
Plasmid#167578PurposeExpress the ATP-insensitive control sensor GRAB_ATP1.0mut in neuronsDepositorInsertATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMJA284= pSin-Pur-MCS-Puro
Plasmid#116877PurposeLentiviral transfer vector containing a multible cloning siteDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
eGFP Y93H TC editing reporter
Plasmid#198882PurposeExpress eGFP fluorescence after Y93H correction and mCherry as transfection controlDepositorInsertenhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationY93HPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB9336 (pgRNA_II-1_NatMX)
Plasmid#161589PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site II-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rap-MESA-20-M-Gal4
Plasmid#84554PurposeMESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivatorDepositorInsertRap-MESA-FRB-20-M-Gal4
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK4_002
Plasmid#123811PurposeEncodes SV40 polyadenylation signal as a Type 4 part to be used in the MTK systemDepositorInsertSV40 pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Expression scaffold for dCas9+sgRNA
Plasmid#171799PurposePICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNADepositorInsertPICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET20b-NLuc-MBP-6xHis
Plasmid#135931PurposeExpresses NanoLuciferase in bacterial cells.DepositorInsertNanoLuciferase
UseTagsMBP-6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWXLd-RIEP-GpNLuc
Plasmid#135935PurposeExpresses the GpNLuc LumiFluor in mammalian cells.DepositorInsertGpNLuc LumiFluor
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>QF2(newHA1)
Plasmid#104873PurposeHACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of insertsDepositorInsertT2A-QF2
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
WT TXNIP-FLAG
Plasmid#200091PurposeWT human TXNIP with C-terminal 3xFLAG tag in pQCXIP vectorDepositorInsertTXNIP (TXNIP Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-4
Plasmid#160704PurposeTrichoderma optimised Synthetic mCherry bordered by BsaI restriction sitesDepositorInsertoptimised synthetic mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only