-
Plasmid#191175PurposeMammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).DepositorInsertCMV-TO:mCherry:s70587:EGFP (frameshifted)
UseTagsExpressionMammalianMutationEGFP is frameshiftedPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13(+9A)-IDR-mCherry-CRY2-NLS
Plasmid#145263PurposeMammalian Expression of Nuclear Opto HOXD13(+9A) IDR with SV40 NLSDepositorInsertHOXD13(+9A)-IDR (HOXD13 Human)
UseTagsExpressionMammalianMutationA-repeat expandedPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHBS1527 [IBB-GFP-mCherry3E]-[BFP-TDP43 W-S]
Plasmid#133333PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll W to S in CTD of full-length TDP43PromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-CRY2oligo-mCherry-ANXA11(186-505 aa)
Plasmid#164208PurposeExpresses CRY2oligo-mcherry tagged ANXA11 C terminal under CMV promoterDepositorInsertANXA11 (ANXA11 Human)
UseTagsCRY2oligo-mcherryExpressionMammalianMutation186-505 aaPromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Linker-hSyn::mCherry.3xFLAG-WPRE
Plasmid#120391PurposepAAV plasmid expressing mCherry.3XFLAG under the hSyn promoter with a Linker containing BstBI, BspQI and SacI sites upstream of the hSyn promoterDepositorTypeEmpty backboneUseAAVTagsmCherry.3XFLAGExpressionMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
AktPH-mCherry-T2A-PHR-T2A-CIBNcaax
Plasmid#173861PurposeMammalian expression of Akt PH-mCherry-T2A-PHR-T2A-CIBNcaax (PIP3 marker and opto-control)DepositorInsertAktPH-mCherry-T2A-PHR-T2A-CIBNcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCAGAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry-T2A-miniSOG-C-Far
Plasmid#193915PurposeExpresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV productionDepositorInsertminiSOG
UseAAVTagsFarnesylation signal sequence and Flag tagExpressionMammalianMutationPromoterhSynAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC119- Padh81-GBP-mCherry(C)-hphMX6-lys1*
Plasmid#89071Purposegenomic GBP-mCherry epitope C-terminal tagging at lys1+ locus in S. pombeDepositorTypeEmpty backboneUseGenomic integration in s. pombeTagsGBP-mCherryExpressionYeastMutationPromoterAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - mCherry - BirA*
Plasmid#131136PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-mCherry-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192242Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3fDepositorInserteIF3f-knockin-homology arm-mAID-mCherry2-Hygro (EIF3F Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
EK0289 SFFV-mCherry-SG(s17d9b)SG-EGFP (FLP-IN)
Plasmid#191142PurposeExpression of Sensor 2 (for EK0211) in mammalian cells; mCherry marker, EGFP output.DepositorInsertmCherry:s17d9b:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2
Plasmid#91873PurposePhoto-activatable/light sensitive Vav2DepositorInsertsUseTagsmCherryExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPRE
Plasmid#120393PurposepAAV plasmid expressing Diras2 shRNA1 under the U6 promoter and mCherry.3XFLAG under the hSyn promoterDepositorInsertDiras2 shRNA (Diras2 Mouse)
UseAAV and RNAiTagsmCherry.3XFLAGExpressionMutationPromoterU6Available sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670)
Plasmid#100061PurposeAAV expression of ChR1-VChR1 Chimera fused to mCherry driven by Ef1a promoterDepositorHas ServiceAAV9InsertChR1-VChR1 Chimera
UseAAVTagsmCherryExpressionMammalianMutationE122T and E162TPromoterEF1aAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ttx-3-mCherry-SL2-Post-SPHERE-SF-iGluSnFR
Plasmid#187100PurposeGlutamate sensor for localization to membrane contatctDepositorArticleInsertPost-SPHERE-SF-iGluSnFR
UseTagsmCherry-SL2ExpressionWormMutationPromoterTtx-3Available sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]
Plasmid#168169PurposeExpression of YFP-BeCyclOp(A-2x) in BWMs of C. elegansDepositorInsertYFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
UseTagsYFPExpressionWormMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-mCherry
Plasmid#75133PurposeRetroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)DepositorInsertshBrd9_1116
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWPT-ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49229PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8
Plasmid#129048Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA8 (huTSDR)
UseTagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1
Plasmid#129041Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA1 (huTSDR)
UseTagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only