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pYEXC3GH

Plasmid

#49027

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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p6042 pcDNA-Xpress-His-USP5

Plasmid

#24744

Depositor

Peter Howley

Article

Vos et al J Virol. 2009 Sep . 83(17):8885-92.

Insert

USP5 (USP5 Human)

Use

Tags

His and Xpress

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2010

Availability

Academic Institutions and Nonprofits only
pDSAY

Plasmid

#62291

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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BKAR-T/A

Plasmid

#14876

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

T/A

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pLentiN 11E6

Plasmid

#37448

Purpose

Lentiviral expression of HPV 11E6 in mammalian cells

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 11E6

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
pICXC3H

Plasmid

#49022

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pJA258

Plasmid

#21511

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

(Gly)5Ala::citrine(w introns)::tbb-2 3'UTR

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pDSAP

Plasmid

#62293

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, Puromycin resistance marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pRSETB-roGFP1-R10

Plasmid

#83310

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Cannon et al Protein Sci. 2006 Jan;15(1):45-57. Epub

Insert

roGFP1-R10

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/Q80R/S147C/Q204C/F223R/S202K/K41D

Promoter

T7

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pUC19-THG73tRNA

Plasmid

#21292

Depositor

Henry Lester

Article

Saks et al J Biol Chem. 1996 Sep 20. 271(38):23169-

Insert

T. thermophila tRNA Glutamine (CUA)-G73

Use

T. thermophila

Tags

Expression

Mutation

N/A

Promoter

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP21

Plasmid

#80675

Purpose

Encodes the synthetic coiled coil peptide SYNZIP21

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP21

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAL198

Plasmid

#22281

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

p!SV40-mCherry-WaspGBD

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNAI Tat C30G HA (P#38)

Plasmid

#14656

Depositor

Matija Peterlin

Article

Cujec et al Mol Cell Biol. 1997 Apr . 17(4):1817-23.

Insert

Tat (tat HIV)

Use

Tags

HA

Expression

Mammalian

Mutation

C30G C34G

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pENTR4-V5-2 (w234-1)

Plasmid

#17426

Purpose

Gateway entry vector for N-term V5 tag, frame B

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP18

Plasmid

#80672

Purpose

Encodes the synthetic coiled coil peptide SYNZIP18

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP18

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pYEXNH3

Plasmid

#49024

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pBad-HPII TAG392

Plasmid

#105848

Purpose

expresses E. coli HPII catalase with TAG stop codon at site 392 and N-terminal 6x his tag

Depositor

Ryan Mehl

Article

Mehl et al 2020

Insert

hydroperoxidase II catalase

Use

Tags

6x his tag

Expression

Bacterial

Mutation

TAG stop codon at site 392

Promoter

pBad/arabinose

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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Pentaprobe PP5

Plasmid

#83330

Purpose

Pentaprobe for assesing the RNA binding potential of a protein

Depositor

Joel Mackay

Article

Bendak et al Nucleic Acids Res. 2012 Aug;40(14):e105.

Insert

PP5

Use

Rna transcription

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCBFD2Bad

Plasmid

#53574

Purpose

Produces an active CBFD (carotenoid β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

cbfd2

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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Hec1 mCherry Plk1 K82R

Plasmid

#45206

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Hec1-targeted mCherry Plk1 K82R

Use

Tags

Expression

Mammalian

Mutation

Plk1 K82R mutation

Promoter

T7

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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