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Plasmid#193562PurposeExpresses human Clathrin Light Chain B truncation mutant (deleted 1-89 aa) tagged with mEGFP in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
UseTagsmEGFPExpressionMammalianMutationDeleted amino acids 1-89PromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
H2A-mTurquoise-CDC42-G12V-deltaCAAX
Plasmid#176094PurposeConstitutively active, nuclear localized Cdc42DepositorInsertCDC42 (CDC42 Human)
UseTagsExpressionMammalianMutationG12VPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585_L139*
Plasmid#174400PurposeS. Typhimurium gene stm2585 (sarA/steE) truncated after 139th amino acid, which removes the stretch homologous to gp130DepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationL139* (Truncation after 139th amino acid in sarA)PromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_∆R13–DD-talin1 (1-2299)
Plasmid#166125PurposeFor expression of mouse talin-1 (residues 1-2299) with C-terminal deletion of dimerization domain and R13 (∆R13-DD) in mammalian cells. Contains C-terminal EGFP tag.DepositorInsert∆R13–DD-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationresidues 1-2299, deletion of dimerization domain …PromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tac(5A)-GFP
Plasmid#162495PurposeExpresses C-terminally GFP tagged Tac with mutated five O-glycosylation sites in mammalian cellsDepositorInsertTac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
UseTagsGFPExpressionMammalianMutationThe five threonine residues are mutated into alan…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tac(5A)
Plasmid#162493PurposeExpresses GFP tagged Tac with mutated five O-glycosylation sites in mammalian cellsDepositorInsertTac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
UseTagsGFPExpressionMammalianMutationThe five threonine residues are mutated into alan…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tac-STC
Plasmid#162490PurposeExpresses partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cellsDepositorInsertpartial length Tac (stem region, TMD and cytosolic tail) (IL2RA Human)
UseTagsGFPExpressionMammalianMutationTac is in partial length with its stem region, TM…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Ndufa2-g1)-PGKpuroBFP-W
Plasmid#105030PurposeLentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFPDepositorInsertNdufa2 (Ndufa2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Ndufa2-g2)-PGKpuroBFP-W
Plasmid#105031PurposeLentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFPDepositorInsertNdufa2 (Ndufa2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His
Plasmid#83457PurposeHuman PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632)DepositorInsertPolycystin-1 (PKD1 Human)
UseTags6xHis and MycExpressionMammalianMutationAmino-acids 4127-4303PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-DroshaK382R
Plasmid#62519PurposeLysine 382 of Drosha was mutated to arginineDepositorInserthuman Drosha with lysine 382 changed to arginine (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged lysine 383 to argininePromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-CAF1-C-10
Plasmid#57784PurposeLocalization: Chromatin Assembly Factor 1, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertCAF1
UseTagsPA-mCherry-miniSOGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-imp α
Plasmid#119719PurposeExpresses mCherry-tagged human importinα in mammalian cellsDepositorInsertimportinα (KPNA2 Human)
UseTagsmCherryExpressionMammalianMutationcontains amino acids 251-529PromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
SspB-HaloTag-DHPH-ITSN1
Plasmid#176113PurposeHeterodimerization with iLID, Cdc42 activationDepositorInsertITSN1 (ITSN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Gal3
Plasmid#85662PurposeFluorescent reporter for lysosomal damageDepositorInsertLGALS3 (LGALS3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-DroshaS300E/S302D
Plasmid#62531PurposeSerine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acidDepositorInserthuman Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged serine 300 to glutamic acid and serine 3…PromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hGal3
Plasmid#73080PurposeExpression in mammalian cellsDepositorInsertGalectin-3 (LGALS3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-GRAM-W
Plasmid#211701PurposeExpression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (EGFP-GRAM1b G187W) in mammalian cellsDepositorInsertGRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseTagsEGFPExpressionMammalianMutationChanged Glycine 187 to TryptophanPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-E-Syt1
Plasmid#66830PurposeMammalian Expression, Bacterial ExpressionDepositorInsertE-Syt1 (ESYT1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -