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Plasmid#223957PurposeGateway cloning of genes of interest with an N-terminal V5-mEGFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseLentiviralTagsV5-mEGFPExpressionMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only
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DG SaCas9 GFP V3
Plasmid#226961PurposeCBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
SaCas9 GFP V3
Plasmid#226962PurposeCBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_λHA-GFPopt
Plasmid#210575PurposeExpresses lambdaHA-optimal GFPDepositorInsertLambdaHA-GFP optimal
UseTagslambda HAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_λHA-GFPnonopt
Plasmid#210576PurposeExpresses lambdaHA-nonoptimal GFPDepositorInsertLambdaHA-GFP nonoptimal
UseTagslambda HAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE81XN-moxGFP2
Plasmid#218962PurposeExpresses Hisx6-Tagged moxGFP2 in bacteriaDepositorInsertmoxGFP2
UseTags6X-HisExpressionBacterialMutationPromoterT5Available sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-eGFP
Plasmid#221710PurposeExpression of eGFP in zebrafish embryos.DepositorInserteGFP
UseFish expressionTagsExpressionMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1 MOSPD3
Plasmid#226405PurposeExpression of human MOSPD3 fused to EGFP in mammalian cellsDepositorInsertMOSPD3 (MOSPD3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1 MOSPD1
Plasmid#226404PurposeExpression of human MOSPD1 fused to EGFP in mammalian cellsDepositorInsertMOSPD1 (MOSPD1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-MLC2 WT
Plasmid#195235PurposeMammalian expression of GFP tagged MLC2. Wild-typeDepositorInsertMLC2 (MYL2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVUT-eC3GFP
Plasmid#225485PurposeTet-on lentiviral vector for controlled expression of C. botulinum exoenzyme C3 transferase and EGFPDepositorInsertExoenzyme C3 transferase
UseLentiviralTagsEGFPExpressionMammalianMutationAmino acids 30-251, See Depositor CommentsPromoterHuman ubiquitin CAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF13AS1371A
Plasmid#224409PurposeMammalian expression of KIF 13a S1371ADepositorInsertKIF 13a S1371A (Kif13a Mouse)
UseTagsGFPExpressionMammalianMutationS1371APromoterChicken Beta actinAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF13AWLDLE
Plasmid#224410PurposeMammalian expression of KIF 13a WLDLEDepositorInsertKIF 13a WLDLE (Kif13a Mouse)
UseTagsGFPExpressionMammalianMutationWLDLEPromoterChicken Beta actinAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_GFP-TEV
Plasmid#223881Purpose4G-cloning donor for N-terminal GFP-TEV tagDepositorInsertGFP-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_3C-GFP
Plasmid#223888Purpose4G-cloning donor for C-terminal 3C-GFP tagDepositorInsert3C-GFP
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-VCF1 WT
Plasmid#223018PurposeExpression of GFP-tagged VCF1 in mammalian cells.DepositorInsertVCF1 (VCF1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMV/TetO2Available sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
peGFP NOVA2 op
Plasmid#224354PurposeExpress GFP-tagged human NOVA2 (codon optimized)DepositorInsertNOVA2 (NOVA2 Human)
UseTagseGFPExpressionMammalianMutationcodon optimized for expression in human cellsPromoterCMV and T7Available sinceSept. 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
EGFP-INPP5E
Plasmid#223564PurposeExpresses the full-length mouse INPP5E (Pharbin)DepositorInsertINPP5E (Inpp5e Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVUT-spGFP
Plasmid#225486PurposeTet-on lentiviral vector for controlled expression of secretable and permeable EGFPDepositorInsertIgκ leader and TAT peptide
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterHuman ubiquitin CAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVUT-spC3GFP
Plasmid#225487PurposeTet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFPDepositorInsertSecretable and permeable exoenzyme C3 transferase
UseLentiviralTagsEGFPExpressionMammalianMutationAmino acids 30-251PromoterHuman ubiquitin CAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only