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Plasmid#194678PurposeCMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFPDepositorInsertCHRM3_cpHalo∆
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Myc-Oct3/4-IP
Plasmid#13460DepositorInsertOct3/4 (Pou5f1 Mouse)
UseTagsMyc (6x)ExpressionMammalianMutationNonePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Hyg
Plasmid#60508PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP
Plasmid#85428PurposeLentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expressionDepositorInserthuman IL-12
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMito-Electra2
Plasmid#184927PurposeMitochondrial labeling with blue fluorescent protein Electra2 fused to partial COX8ADepositorInsertCOX8A-Electra2
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-FLucStop
Plasmid#177617PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).DepositorInsertSARS-CoV-2 FSE
UseLuciferaseTagsExpressionMammalianMutationIn-frame stop codon added between FLuc and FSEPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-tdTomato (codon diversified)
Plasmid#59462PurposeAAV-mediated expression of tdTomato (codon diversified) under the CAG promoterDepositorHas ServiceAAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserttdTomato
UseAAVTagsExpressionMammalianMutationcodon diversifiedPromoterCAGAvailable sinceSept. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Human IntS6L
Plasmid#198409PurposeExpresses FLAG-tagged human IntS6LDepositorInsertIntegrator complex subunit 6 like (INTS6L Human)
UseTags3x FLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CaaX(Hras)
Plasmid#108886PurposeExpression of membrane localized mCherryDepositorInsertmCherry-CaaX(Hras)
UseCan be used for mammalian expression or in vitro …TagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FRT-Rev-3xGFP
Plasmid#191204PurposeFlpO dependent GFP expressionDepositorInsert3X GFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v6 (fl)
Plasmid#137816Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v6 (fl) (CD44 Human)
UseTagsGFPExpressionMammalianMutationR296K in the V6 region [Please see depositor comm…PromoterPGKAvailable sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVUT-tTR-KRAB
Plasmid#11651PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generationDepositorHas ServiceCloning Grade DNAInserthUbiquitin C, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
hrGFP-Keap1
Plasmid#28025DepositorInsertKeap1 (KEAP1 Human)
UseTagshrGFPExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
Nuclear PancACe
Plasmid#215706PurposeExpresses acetyl-CoA biosensor in the nucleusDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and NLSExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BFP
Plasmid#25891DepositorInsertBFP
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ 2xFLAG-2xSTREP_BACH1
Plasmid#159130Purposeexpresses BACH1 in mammalian cellsDepositorInsertBACH1 (BACH1 Human)
UseTags2X-FLAG _ 2X-STREPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-mNeonGreen-EC18153
Plasmid#137075PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE7.10
Plasmid#102919Purposeexpresses ABE7.10 in mammalian cellsDepositorInsertABE7.10
UseTagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mito-GA
Plasmid#209865PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVSDepositorInsertddGFP A
UseTagsTargeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLT3-V5/HIS
Plasmid#201985Purposeexpression of human FLT3 receptor tyrosine kinase in mammalian cellsDepositorInsertFLT3 receptor tyrosine kinase (FLT3 Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_COCVmut_G
Plasmid#207262PurposeMammalian expression of a mutant Cocal virus G proteinDepositorInsertCOCVmut_G
UseTagsExpressionMammalianMutationK47A/Y209A/R354A; mammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDY1314_RADARS_Luciferase
Plasmid#193191PurposeExpress Gaussia Luciferase conditional upon RNA transcriptDepositorInsertRADARS Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-BsdCas9-W
Plasmid#67978PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termi…ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX311-GFP-MEKDD
Plasmid#194882PurposeExpression of dominant negative MEK1DepositorInsertMEKDD (MAP2K1 Human)
UseLentiviralTagsExpressionMammalianMutationS218D, S222DPromoterE1FaAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-vhhGFP4-hIgG1-Fc
Plasmid#105576PurposeT7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusionDepositorInsertHuman IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-TOP-dGFP-reporter
Plasmid#14715PurposeBeta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).DepositorInsertLEF-1/TCF-responsive promoter (Ctnnb1 )
UseLentiviralTagsd2 eGFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
NLS-mCherry-LEXY (pDN122)
Plasmid#72655PurposeBlue light-inducible nuclear export of mCherryDepositorInsertNLS-mCherry-LEXY
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
LeGO-dKatushka2
Plasmid#85214PurposeLentiviral vector expressing dKatushka2 fluorescent protein driven by SFFV promoterDepositorInsertdKatushka2
UseCre/Lox and LentiviralTagsExpressionMammalianMutationBsrGI site removed at 5' end, BsrGI site ins…PromoterSFFVAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCVhygro-F-G9a
Plasmid#41721PurposeRetroviral expression of human G9a in mammalian cellsDepositorInsertG9a (EHMT2 Human)
UseRetroviralTagsflagExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagC 75L
Plasmid#19305DepositorInsertRas-related GTP binding C (RRAGC Human)
UseTagsGST and HAExpressionMammalianMutationS75LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only