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Plasmid#170917PurposeExpresses FLAG-BirA-eGFP-NLS2 to be used as control in BioID or FLAG pull downDepositorInsertFLAG-BirA-eGFP-NLS2
UseTagsBirA-FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HR-BirA-FLAG_Ago2
Plasmid#170918PurposeEncodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago2DepositorInsertBirA-FLAG
UseTagsExpressionMammalianMutationPromoterlacAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HR-BirA-FLAG_Ago1
Plasmid#170919PurposeEncodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago1DepositorInsertBirA-FLAG
UseTagsExpressionMammalianMutationPromoterlacAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-zfCSF3b
Plasmid#168202PurposeExpress zebrafish Csf3b, mRNA synthesisDepositorInsertCsf3b (csf3b Zebrafish)
UseVertebrate expression (zebrafish, chicken, xenopu…TagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B K88E
Plasmid#175028PurposeExpresses WIPI2B (K88E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorInsertWIPI2B (K88E) (WIPI2 Human)
UseTagsHaloTagExpressionMammalianMutationLysine 88 to Glutamate (AAG to GAG)PromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B I92E
Plasmid#175029PurposeExpresses WIPI2B (I92E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorInsertWIPI2B (I92E) (WIPI2 Human)
UseTagsHaloTagExpressionMammalianMutationIsoleucine 92 to Glutamate (ATC to GAA)PromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B C93E
Plasmid#175030PurposeExpresses WIPI2B (C93E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorInsertWIPI2B (C93E) (WIPI2 Human)
UseTagsHaloTagExpressionMammalianMutationCysteine 93 to Glutamate (TGC to GAG)PromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B K128E
Plasmid#175031PurposeExpresses WIPI2B (K128E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorInsertWIPI2B (K128E) (WIPI2 Human)
UseTagsHaloTagExpressionMammalianMutationLysine 128 to Glutamate (AAG to GAG)PromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP71-mGFP-LLO-hPMEL
Plasmid#174607Purposeexpresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the human hPMEL epitopeDepositorInsertexpresses palmitoylated GFP w/C-term fusion of the LLO190 epitope and human hPMEL epitope
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (E60amb)-His
Plasmid#168043PurposeExpresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationGlu 60 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK129-stm2585_Y167F
Plasmid#174402PurposeBacterial expression of sarA with its STAT3-binding YxxQ motif mutated to FxxQDepositorInsertsarA
UseTagsExpressionBacterialMutationsarA with Y167F mutationPromoterEndogenousAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-zfCSF3a
Plasmid#168201PurposeExpress zebrafish Csf3a, mRNA synthesisDepositorInsertCsf3a (csf3a Zebrafish)
UseVertebrate expression (zebrafish, chicken, xenopu…TagsExpressionMammalianMutationDeletion of A27PromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (E143amb)-His
Plasmid#168046PurposeExpresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationGlu 143 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (S87amb)-His
Plasmid#168044PurposeExpresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationSer 87 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N116amb)-His
Plasmid#168045PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationAsn 116 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N21amb)-His
Plasmid#168041PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationAsn 21 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgβ-anticalin-His-ybbr
Plasmid#168040PurposeExpresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminusDepositorInsertAnticalin
UseTags6x Histag, Fgβ peptide, and ybbr tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (I55amb)-His
Plasmid#168042PurposeExpresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationIle 55 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTMP
Plasmid#129560Purposecytoplasmic markerDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-mu24 1-181-2xStrep
Plasmid#138444PurposeExpresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertmu24
UseTagsStrepExpressionMammalianMutationAmino acids 1-181PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only