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pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

Plasmid

#128755

Purpose

Expresses CYRANO gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

CYRANO sgRNA (OIP5-AS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup35-EcoRI

Plasmid

#87332

Purpose

To express shRNA against human Nup35

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup35

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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p667-UBC-DYRK3-V5-miniTurbo_IDG-K

Plasmid

#158190

Purpose

Gateway destination clone of DYRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

DYRK3-V5-miniTurbo (DYRK3 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

R293H- please see depositor comments

Promoter

Ubiquitin

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRb_candidate1

Plasmid

#164996

Purpose

Expression of candidate TCR #1 TCRbeta cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRB (TRB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate1

Plasmid

#164995

Purpose

Expression of candidate TCR #1 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1 Flag-Sesn2

Plasmid

#61871

Purpose

Lentivirial expression of Flag-Sesn2 (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn2 (Sesn2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
pMXs-EGFP-Rheb SSVM-IP

Plasmid

#13832

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

Rheb SSVM (Rheb Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C181 changed to serine

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pLPC myc-mRAP1

Plasmid

#73136

Purpose

Expression of myc-tagged mRAP1

Depositor

Titia de Lange, Agnel Sfeir

Article

Sfeir et al Science. 2010 Mar 26;327(5973):1657-61.

Insert

Rap1 (Terf2ip Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Orai1-myc E106D MO70

Plasmid

#22755

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

Orai1 E106D (ORAI1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

E106D

Promoter

Available since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pBABEPuro-rnSOX9

Plasmid

#63009

Purpose

Expresses rat SOX9

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

SOX9 (Sox9 Rat)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pCXCR4_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101114

Purpose

Encodes dCas9(N) fused to CXCR4

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

CXCR4-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup133-2

Plasmid

#87333

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMIR-R/Tac1/SG

Plasmid

#16296

Depositor

Pranela Rameshwar

Article

Greco et al Proc Natl Acad Sci U S A. 2007 Sep 25. 1

Insert

Tac1 3'UTR (TAC1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pLPC FLAG hRap1 delta Br delta M

Plasmid

#13256

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Br delta M (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

contains hRap1 aa2-44 + 184-399 and the point mut…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pSUPERIOR.puro-shREV3-4

Plasmid

#38029

Depositor

Rolf Stahel, Emanuela Felley-Bosco

Article

Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

Insert

shREV3-4 (REV3L Mouse, human, Human, Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pX459V2.0+K848A

Plasmid

#108296

Purpose

pX459 V2.0 (Plasmid #62988) with the K848A mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CAMK1D_IDG-K

Plasmid

#158167

Purpose

Gateway destination clone of CAMK1D (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CAMK1D (CAMK1D Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRb_candidate10

Plasmid

#165008

Purpose

Expression of candidate TCR #10 TCRbeta cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRB (TRB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTCRb_candidate6

Plasmid

#165002

Purpose

Expression of candidate TCR #6 TCRbeta cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRB (TRB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTCRa_candidate6

Plasmid

#165001

Purpose

Expression of candidate TCR #6 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge