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We narrowed to 3,890 results for: chm

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pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

Plasmid

#224123

Purpose

Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-mutant (Q20A_Y23A_Y24F_F35A)

Use

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Q20A_Y23A_Y24F_F35A in La(SSB)1-194

Promoter

CMV

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-806_HA-GD2-28z_CAR_RFP-TFAP4

Plasmid

#207493

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-851_HA-GD2-28z_HA-TFAP4

Plasmid

#207501

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

HA-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-V1-CD20-Puro

Plasmid

#209755

Purpose

Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 1 mRNA isoform.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V2-CD20-Puro

Plasmid

#209756

Purpose

Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 2 mRNA isoform.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-kz-CD20-Puro

Plasmid

#209759

Purpose

Lentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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1529_pAAV-CB-FKBP12-BirA-HA

Plasmid

#208200

Purpose

Express the murine FKBP12-BirA-HA fusion protein in AAV vector

Depositor

Susan Hamilton

Article

Lee et al Commun Biol. 2023 Sep 14;6(1):942. doi:

Insert

Fkbp12 (Fkbp1a Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

Chicken beta-actin

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_1kb_KLF2mut 732

Plasmid

#194195

Purpose

Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 732)

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Tags

Expression

Mutation

mutated KLF binding sites (position 732)

Promoter

SMANTIS promoter (1kb), mutated

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_1kb_KLF2mut 884

Plasmid

#194196

Purpose

Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 884)

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Tags

Expression

Mutation

mutated KLF binding sites (position 884)

Promoter

SMANTIS promoter (1kb), mutated

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_1kb_KLF2mut 449

Plasmid

#194197

Purpose

Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 449)

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Tags

Expression

Mutation

mutated KLF binding sites (position 449)

Promoter

SMANTIS promoter (1kb), mutated

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899 (Quin)

Plasmid

#194191

Purpose

Includes the promoter (1kb) of SMTS with 4 mutated KLF binding sites (positions 449,548,732,899)

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Tags

Expression

Mutation

4 mutated KLF binding sites (positions 449,548,73…

Promoter

SMANTIS promoter (1kb), mutated

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_1kb_KLF2mut 449,548,732 (Tri)

Plasmid

#194192

Purpose

Includes the promoter (1kb) of SMTS with 3 mutated KLF binding sites (positions 449,548,732)

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Tags

Expression

Mutation

3 mutated KLF binding sites (positions 449,548,73…

Promoter

SMANTIS promoter (1kb), mutated

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_1kb_KLF2mut 548

Plasmid

#194194

Purpose

Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 548)

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Tags

Expression

Mutation

mutated KLF binding sites (position 548)

Promoter

SMANTIS promoter (1kb), mutated

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pInducer-FRA1

Plasmid

#192267

Purpose

Doxycycline-inducible expression of murine FRA1 (Fosl1)

Depositor

Günter Schneider

Article

Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

Insert

Fosl1 (Fosl1 Mouse)

Use

Lentiviral; Inducible expression

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 C-V5-tag ORF7a

Plasmid

#179971

Purpose

Mammalian expression vector for SARS-CoV-1 ORF7a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 ORF7a (ORF7a Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 NSP7 C-V5-tag

Plasmid

#179963

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp7, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 Nsp7 (ORF1ab Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_MECP2_MBD-R133C

Plasmid

#179409

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R133C)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD(R133C) (Mecp2 Mouse)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD R133C

Promoter

CAGGS

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_MECP2_MBD-T158M

Plasmid

#179410

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(T158M)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD(T158M) (Mecp2 Mouse)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD T158M

Promoter

CAGGS

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LeGO-iBSD-5LO-p12

Plasmid

#182744

Purpose

Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform p12) in mamallian cells with blasticidin S resistance (bsd).

Depositor

Dieter Steinhilber

Article

Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

Insert

5-Lipoxygenase isoform p12 (ALOX5 Human)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

codon optimized for expression in homo sapiens

Promoter

SFFV

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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LeGO-iBSD-5LO-E376Q

Plasmid

#182742

Purpose

Lentiviral expression vector for codon-optimized 5-Lipoxygenase (enzymatically inactive E376Q mutant) in mamallian cells with blasticidin S resistance (bsd).

Depositor

Dieter Steinhilber

Article

Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

Insert

5-Lipoxygenase E376Q (ALOX5 Human)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

enzymatically inactive 5-LO E376Q mutant, codon o…

Promoter

SFFV

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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