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Showing: 3261 - 3280 of 6709 results

  6. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

    Plasmid
    #140204
    Purpose
    Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Step 1 of cloning c…
    Tags
    Expression
    Mutation
    Promoter
    Aspergillus fumigatus U3 (RNAPIII).
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    140204-plasmid-map-sequence-id-309884

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  8. pFRT-TODestFLAGHA_RBPMS_KDR_HindIII

    Plasmid
    #65662
    Purpose
    FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)
    Depositor
    Thomas Tuschl
    Article
    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.
    Insert
    RBPMS (RBPMS Human)
    Use
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    introduced HindIII site (final goal to exchange F…
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65662-plasmid-map-sequence-id-111633

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  9. pG418R-MCS-QF-MiniWhite

    Plasmid
    #165902
    Purpose
    G418 resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable Empty Enhancer Driver
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    165902-plasmid-map-sequence-id-328281

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  10. Cx26-sfGFP

    Plasmid
    #69026
    Purpose
    Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to non-monomerized superfolder GFP. Expression driven by CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx26 (Gjb2 Rat)
    Use
    Tags
    non-monomerized superfolder GFP (V206)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    69026-plasmid-map-sequence-id-124714

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  15. pLpC-Zipp.-L-IFP-F[1]+Plum (mammalian)

    Plasmid
    #52907
    Purpose
    Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[1]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI. Confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Zipp.-L-IFP-F[1]+Plum
    Use
    Retroviral
    Tags
    L-IFP-F[1]
    Expression
    Mammalian
    Mutation
    Promoter
    Unknown
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 3261 - 3280 of 6709 results