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pScalps_puro_mklf1

Plasmid

#59918

Purpose

Expresses mouse Kruppel-like factor1

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

Kruppel-like factor 1 (Klf1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
p53-(1-393)-E285V-N268D

Plasmid

#35088

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-393 - E285V-N268D

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pPD95.75-cYAP-1

Plasmid

#66857

Purpose

Generate transgenic C.elegans with cYAP-1 fused to GFP

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

C.elegans YAP-1 (yap-1 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Serine104 is mutated to alanine

Promoter

C. elegans 5'UTR

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pPD95.75-cYAP-1 S104A

Plasmid

#66858

Purpose

Generate transgenic C.elegans with cYAP1- fused to GFP, in which Serine 104 is mutated to alanine

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

YAP-1 (yap-1 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Serine104 is mutated to alanine

Promoter

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
p6615 MSCV-IP N-HAonly NIPA1

Plasmid

#35013

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

NIPA1 (RBCK1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

Feb. 9, 2012

Availability

Academic Institutions and Nonprofits only
pX330_NPM2_cterm1

Plasmid

#222911

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 1 (NPM2 Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NPM2_cterm4

Plasmid

#222914

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 4 (NPM2 Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NPM2_cterm3

Plasmid

#222913

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 3 (NPM2 Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR2_TFAP2C-T2A-mGreenLantern-Hygro

Plasmid

#222915

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to TFAP2C.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NPM2_cterm2

Plasmid

#222912

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 2 (NPM2 Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-hTBP

Plasmid

#171073

Purpose

Expresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBP

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

TATA-box-binding protein (TBP Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET41SIII-ATF6(1-373)

Plasmid

#171074

Purpose

Expresses of ATF6α(1-373) in bacteria

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

ATF6α (ATF6 Human)

Use

Tags

GST and His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-NEUROD1

Plasmid

#215031

Purpose

Mammalian expression of human NEUROD1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

neuronal differentiation 1 (NEUROD1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro

Plasmid

#189634

Purpose

Lentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

hU6 Cas9-Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRS193

Plasmid

#197143

Purpose

scCloneSelect C-to-T DNA barcode cloning backbone (EF1a promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRS138

Plasmid

#197142

Purpose

scCloneSelect C-to-T DNA barcode cloning backbone (CMV promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pWP052

Plasmid

#198774

Purpose

Drives specific expression in the ALN, PLN neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pnlp-20-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pWP003

Plasmid

#198803

Purpose

Drives specific expression in the URX neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pflp-8-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pWP022

Plasmid

#198798

Purpose

Drives specific expression in the PHA neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psrg-13-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pWP172

Plasmid

#198796

Purpose

Co-expression plasmid for the OLL neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pseb-3-NLS-gp41-1-C-intein-cGAL(AD)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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