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Plasmid#200213PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 H25A mutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-458
Plasmid#200214PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 E184A mutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-459
Plasmid#200215PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 K214A mutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-460
Plasmid#200216PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 R55E-N56D mutationsPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-461
Plasmid#200217PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 K11D-R12E mutationsPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-462
Plasmid#200218PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 K11D-R12E, R55E-N56D mutationsPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trLAT-P
Plasmid#203747PurposeEncodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. The palmitoylation site at C27 is mutated to A. Used as a fluorescent plasma membrane probe.DepositorInserttrLAToC27A (Lat Mouse)
UseTagsER translocation signal - mEos3.2ExpressionMammalianMutationC27APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
trLATm1PtoLmEos3.2
Plasmid#203749PurposeEncodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Prolines P8 and P17 in the LAT transmembrane domain mutated to L. Used as a fluorescent plasma membrane probe.DepositorInserttrLATm1 (PtoL) (LAT Human)
UseTagsmEos3.2ExpressionMammalianMutationP8L, P17LPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-GFP-RELA K5Q
Plasmid#196632PurposeExpress GFP-RELA K5QDepositorInsertGFP-RELA K5Q (RELA Human)
UseLentiviralTagsGFPExpressionMutationK122Q, K123Q, K310Q, K314Q, K315QPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGW02 AAV-TBG-Cre-sgP53-sgRNA
Plasmid#192162PurposeAAV-TBG-Cre-sgP53-sgRNADepositorTypeEmpty backboneUseAAVTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-NT
Plasmid#184838PurposeExpression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.DepositorInsertCo-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+mEGFP-H1.4ΔCTD
Plasmid#157800PurposeExpression of mEGFP fused to human linker histone H1.4 without c-terminal tailDepositorInsertmEGFP-H1.4ΔCTD (H1-4 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+LANA-H1.4CTD
Plasmid#157798PurposeExpression of LANA peptide-human linker histone H1.4 CTD fusion proteinDepositorInsertLANA-H1.4CTD (H1-4 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA BRCA2 R2896C
Plasmid#139511PurposePlasmid expressing a sgRNA to introduce BRCA2 R2896C using base editingDepositorInsertsgRNA to insert BRCA2 E2896C using base editing
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
GS2.1/EC
Plasmid#132568PurposesgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).DepositorInsertegg cell promoter, Cas9-mApple, pds3 sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterA. thaliana egg cell promoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV259
Plasmid#132340PurposeCasD plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasD-AT-CBF1 (CBF1 CasD (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV503
Plasmid#132342PurposeCasE plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasE-AT-CBF1 (CBF1 CasE (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV504
Plasmid#132343PurposeCasA plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasA-AT-CBF1 (CBF1 Mustard Weed, CasA (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana))
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR152
Plasmid#106052PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR152; Opto-GPR152; E6)DepositorInsertOpto-GPR152 (GPR152 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR176
Plasmid#106060PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR176; Opto-GPR176; F2)DepositorInsertOpto-GPR176 (GPR176 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR183
Plasmid#106062PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR183; Opto-GPR183; F4)DepositorInsertOpto-GPR183 (GPR183 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR132
Plasmid#106042PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR132; Opto-GPR132; D8)DepositorInsertOpto-GPR132 (GPR132 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR141
Plasmid#106045PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR141; Opto-GPR141; D11)DepositorInsertOpto-GPR141 (GPR141 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR50
Plasmid#106024PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR50; Opto-GPR50; C2)DepositorInsertOpto-GPR50 (GPR50 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR62
Plasmid#106028PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR62; Opto-GPR62; C6)DepositorInsertOpto-GPR62 (GPR62 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR35
Plasmid#106018PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR35; Opto-GPR35; B8)DepositorInsertOpto-GPR35 (GPR35 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR15
Plasmid#106004PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR15; Opto-GPR15; A6)DepositorInsertOpto-GPR15 (GPR15 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR33
Plasmid#106016PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR33; Opto-GPR33; B6)DepositorInsertOpto-GPR33 (GPR33 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHKU11.1-2.2
Plasmid#115528PurposeExpression of a fungal biosynthetic gene clusterDepositorInsertS_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLacI-GFP-HEC1-d207
Plasmid#114050PurposeExpression of exogenous LacI-GFP-HEC1 delta 207DepositorInsertGFP-HEC1-d207 (NDC80 Human)
UseTagsExpressionMammalianMutationHEC1 delta 207PromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-d207
Plasmid#114051PurposeExpression of exogenous eGFP HEC1 delta 207DepositorInserteGFP-HEC1-d207 (NDC80 Human)
UseTagsExpressionMammalianMutationHEC1 delta 207PromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-M4
Plasmid#114036PurposeExpression of HEC1 M4 mutant tagged with GFPDepositorInsertGFP tagged - HEC1 -M4 (NDC80 Human)
UseTagsExpressionMammalianMutationM4 (K146E, R153E, K156E)PromoterCMV TetOAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-M2
Plasmid#114035PurposeExpression of HEC1 M2 mutant tagged with GFPDepositorInsertGFP tagged - HEC1 -M2 (NDC80 Human)
UseTagsExpressionMammalianMutationM2 (K81E, N87E, K89E)PromoterCMV TetOAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
SMR3B-bio-His
Plasmid#52183PurposeExpresses full-length Submaxillary gland androgen-regulated protein 3B precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSMR3B (SMR3B Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hoxd13-3'UTR
Plasmid#31525DepositorInsertHoxd13-3'UTR (Hoxd13 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUT1147-HQ858691
Plasmid#27737DepositorInsertZmMYB2 transcription factor from AM156904 (myb2 Zea mays)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceMarch 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUT1148-HQ858692
Plasmid#27738DepositorInsertZmMYB8 transcription factor from AM156905 (myb8 Zea mays)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceMarch 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUT1149-HQ858693
Plasmid#27739DepositorInsertZmMYB39 transcription factor from AM156907 (myb39 Zea mays)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceMarch 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUT1150-HQ858694
Plasmid#27740DepositorInsertZmMYB42 transcription factor from AM156908 (myb42 Zea mays)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceMarch 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIS58 TIP120A 3'UTR mut
Plasmid#14502DepositorInsertTIP120A 3'UTR mutant (CAND1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationCATTCC’s were changed to AAGTAC'sPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only