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pcDNA3.1: TfR1-CoilE

Plasmid

#181988

Purpose

CoilE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

TfR1-CoilE

Use

Tags

CoilE

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OKMS-Cas9

Plasmid

#120353

Purpose

piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Insert

OKMS cassette

Use

CRISPR; Piggybac transposon

Tags

Expression

Mammalian

Mutation

Klf4 [10-483]

Promoter

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Par6 S345A

Plasmid

#11749

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

PAR6 (Pard6a Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

S345A

Promoter

Available since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
CSAC-Bmal1

Plasmid

#168296

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgBmal1-1-hU6-sgBmal1-3

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

U6, hSyn

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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sg1+sg2+sg3

Plasmid

#113969

Purpose

Triple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg1+sg2+sg3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJFRC2-INS

Plasmid

#26215

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Aug 9. ():.

Insert

mCD8::GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pBS-MK2

Plasmid

#29637

Depositor

Bruce Tempel

Insert

MK2 (Kcna2 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 5, 2011

Availability

Academic Institutions and Nonprofits only
pSuper-PICK1 shRNA

Plasmid

#67522

Purpose

Expresses shRNA against PICK1

Depositor

Richard Huganir, Victor Anggono

Article

Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

Insert

PICK1 (Pick1, Pick1 Rat, Mouse)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1 Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-012 Vimentin-SuperNova//BsaI

Plasmid

#130193

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

Vimentin-SuperNova

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a-mRor2deltaIg

Plasmid

#22629

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Aug 31. ():.

Insert

Ror2 delta Ig (Ror2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of Ig domain

Promoter

Available since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
pMA2867

Plasmid

#25439

Depositor

Mikhail Alexeyev

Article

Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

Insert

mCherry-miR155-lacZalpha (Mir155hg Discosoma-mouse-E. coli, Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pRVGP-BB

Plasmid

#15380

Depositor

Stephen Smale

Article

Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

Insert

mU6-BRG/BRM shRNA (Smarca4, Smarca2 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-miRFP2

Plasmid

#178968

Purpose

Expresses miRFP2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

miRFP2

Use

AAV

Tags

no

Expression

Mammalian

Mutation

NO

Promoter

CAG

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBS Wnt-1HA

Plasmid

#17367

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-1HA (Wnt1 Mouse)

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pMR150

Plasmid

#32292

Depositor

Verne Chapman, Rudolf Jaenisch

Article

Sanford et al Genes Dev. 1987 Dec;1(10):1039-46.

Insert

genomic methylation probe-minor satellite repeat

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
Anti-GABA(A)R, Alpha4 [N398A/34R]

Plasmid

#177543

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Alpha4 (Rat). Derived from hybridoma N398A/34.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Alpha4 (Rattus norvegicus) recombinant mouse monoclonal antibody (Gabra4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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L1E-1

Plasmid

#21193

Depositor

Paul Khavari

Article

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Insert

Mek1 (Esr1, MAP2K1 Human, Mouse)

Use

Retroviral

Tags

Expression

Mutation

Mek1 R4F fused to ER*, constitutive active

Promoter

Available since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
mCherry-MCPB1-C-18

Plasmid

#55081

Purpose

Localization: Actin Capping Protein , Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

MCPB1

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-MCPB1-N-18

Plasmid

#55082

Purpose

Localization: Actin Capping Protein , Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

MCPB1

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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shMEK1/2 v2 puro

Plasmid

#72568

Purpose

Hairpin targeting MEK1 and MEK2 (murine).

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

MEK1/2 (Map2k1, Map2k2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-sgTp53#2/Cre

Plasmid

#173621

Purpose

Expresses a Tp53-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Tp53 (Trp53 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGL4.24-Cd36-enhancer7

Plasmid

#138579

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17918617_17920400

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Minimal promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.24-Cd36-enhancer6

Plasmid

#138578

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17898458_17899478

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Minimal promoter

Available since

May 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.24-Cd36-enhancer3

Plasmid

#138575

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17848003_17849903

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Minimal promoter

Available since

May 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.24-Cd36-enhancer2

Plasmid

#138574

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17843052_17844983

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Minimal promoter

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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Sema3f(L,+)-AP-His

Plasmid

#72021

Purpose

Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains no deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3f (Sema3f Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-mApg5(K130R)

Plasmid

#22959

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

130 Lysine to Arginine

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pOP462

Plasmid

#85935

Purpose

Encodes the Anti-Ebola 13C6 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

Depositor

Timothy Lu

Article

Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

Insert

Anti-Ebola 13C6 monoclonal antibody

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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PDF1::mCitrine-MBD

Plasmid

#115222

Purpose

Microtubule visualization

Depositor

Jan Traas

Article

Armezzani et al Development. 2018 Jun 4;145(11). pii: de

Insert

Microtubule binding domain

Use

Tags

mCitrine

Expression

Plant

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Nav channel [K58/35R]

Plasmid

#128623

Purpose

Mammalian Expression Plasmid of anti-Pan-Nav channel. Derived from hybridoma K58/35.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Nav Channel Pan recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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