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Addgene
Showing: 3241 - 3260 of 25300 results

  1. pGDP4 ermC

    Plasmid
    #112884
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    ermC (ermM Staphylococcus epidermis)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Plac
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112884-plasmid-map-sequence-id-219861

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  2. pTS2353_Tier3(PB)-YPet_PuroR

    Plasmid
    #169652
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven YPet and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169652-plasmid-map-sequence-id-366196

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  3. pKT3Ts-goldyTALEN

    Plasmid
    #80330
    Purpose
    Updated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequence
    Depositor
    Jeffrey Essner
    Article
    Welker et al Hum Gene Ther. 2016 Jun;27(6):423-4. doi
    Type
    Empty backbone
    Use
    TALEN
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T3
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    80330-plasmid-map-sequence-id-156328

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  11. pJL1-TTlight-4xDQNAT

    Plasmid
    #128394
    Purpose
    In vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Tetanus toxin light chain domain
    Use
    Tags
    4xDQNAT glycosylation tag and 6xHis tag
    Expression
    Bacterial
    Mutation
    Inactivating E234A mutation
    Promoter
    T7
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128394-plasmid-map-sequence-id-260189

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  14. pcDNA3.1(+)MBII-85-5'/3'ss mut

    Plasmid
    #67645
    Purpose
    MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.
    Depositor
    Stefan Stamm
    Article
    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00
    Insert
    MBII85 snoRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5' and 3' splice sites mutations
    Promoter
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    67645-plasmid-map-sequence-id-116914

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  15. pFGL1170R

    Plasmid
    #116896
    Purpose
    hH1:mCherry (nuclear marker)
    Depositor
    Naweed Naqvi
    Article
    Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581
    Inserts
    hH1
    mCherry
    hH1 downstream region
    Use
    Fungal expression (in magnaporthe oryzae)
    Tags
    Expression
    Mutation
    no start codon, no stop codon and without start c…
    Promoter
    promoter less
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    116896-plasmid-map-sequence-id-232119

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  20. pMV-URR2

    Plasmid
    #65332
    Purpose
    standard plasmids for exogenus pathway assembly haboring recombination sequence 2 (Universal Recombination Region 2) which could be released and ligated to series of TUs
    Depositor
    Junbiao Dai
    Article
    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4
    Insert
    URR2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    65332-plasmid-map-sequence-id-137535

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Showing: 3241 - 3260 of 25300 results