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pET22b-ecDHFR-dCys-T46C

Plasmid

#110543

Purpose

Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Use

Tags

Expression

Bacterial

Mutation

T46 mutated to C46, C85 mutated to A85, C152 muta…

Promoter

T7

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-dCys-L54C

Plasmid

#110542

Purpose

Bacterial expression of E.coli DHFR C85A/C152S/L54C mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Use

Tags

Expression

Bacterial

Mutation

L54 mutated to C54, C85 mutated to A85, C152 muta…

Promoter

T7

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-D27S/Y100F

Plasmid

#110827

Purpose

Bacterial expression of E.coli DHFR D27S/Y100F mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

Insert

ecDHFR

Use

Tags

Expression

Bacterial

Mutation

D27 mutated to S27, Y100 mutated to F100

Promoter

T7

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-NmCas9-Flag D16A

Plasmid

#109031

Purpose

D16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Article

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Use

Tags

Flag Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA(R37P)-RagC

Plasmid

#99646

Purpose

Bacterial expression of RagA(R37P)-RagC

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

R37P

Promoter

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA-RagC(K84T))

Plasmid

#99648

Purpose

Bacterial expression of RagA-RagC(K84T)

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

K84T

Promoter

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEThT-MAHS delta MTP

Plasmid

#90032

Purpose

To express His-tagged MAHS in bacteria

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

MAHS

Use

Tags

His

Expression

Bacterial

Mutation

Deletion of mitochondria targeting peptide

Promoter

T7

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pet151D-TOPO-BROX 1-401

Plasmid

#89866

Purpose

Expresses human BROX residues 1-401 in bacteria; internal ID WISP11-297

Depositor

Wesley Sundquist

Article

Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

Insert

BROX (BROX Human)

Use

Tags

His Tag, V5 tag

Expression

Bacterial

Mutation

expresses only residues 1-401

Promoter

T7

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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csd2_121-308,S166A/pET28b(+)

Plasmid

#83301

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: S166A

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Serine 166 …

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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csd2_121-308,T151A/pET28b(+)

Plasmid

#83300

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: T151A

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Threonine 1…

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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csd2_121-308,Y197F/pET28b(+)

Plasmid

#83299

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: Y197F

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Tyrosine 19…

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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csd2_121-308,E165A/pET28b(+)

Plasmid

#83298

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: E165A

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Glutamic ac…

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 D102A, E108A

Plasmid

#74618

Purpose

Expresses a double mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

D102A, E108A

Promoter

T7

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a-Chaetomium thermophilum LIC

Plasmid

#74782

Purpose

encodes dynein light intermediate chain (LIC) from Chaetomium thermophilum; no tag

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

Chaetomium thermophilum dynein light intermediate chain

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pJK040 (pET-PurQ-A128T)

Plasmid

#73840

Purpose

Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

Depositor

JoAnne Stubbe, T. J. Kappock

Article

Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

Insert

formylglycinamidine ribonucleotide synthetase, glutaminase subunit

Use

Tags

Expression

Bacterial

Mutation

A128T mutant

Promoter

T7

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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csd6_17-330/pET28b(+) N-tag

Plasmid

#71353

Purpose

csd6, construct:17-330, pET28b(+) N-tag (Please note that this plasmid has a C-terminal His tag)

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 )

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only
csd6_4-330/pET28b(+) N-tag

Plasmid

#71351

Purpose

csd6, construct:4-330, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 )

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330/pET28b(+) N-tag

Plasmid

#71352

Purpose

csd6, construct:13-330, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 )

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_E110A/pET28b(+) N-tag

Plasmid

#71354

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:E110A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Glutamic acid 110 to Alanine)

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_Y133A/pET28b(+) N-tag

Plasmid

#71356

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:Y133A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Tyrosine 133 to Alanine)

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only