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p-miR-Report + WT miR-181 BS in NLK 3UTR

Plasmid

#22790

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Wild-type miR-181 Binding Site in Nemo-like kinase 3 Untranslated Region (NLK Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
p-miR-Report + MT miR-181 BS in NLK 3UTR

Plasmid

#22791

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Mutant miR-181 Binding Site in Nemo-like kinase 3 Untranslated Region (NLK Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pmirGlo-CALB2-3'UTR delta ARE&mir30

Plasmid

#74430

Purpose

Calb2 UTR with mutated AU binding sequence and mir-30 site 1 and 2

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

Insert

CALB2 3'UTR (CALB2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pINDUCER12 (miR-RUL)

Plasmid

#46935

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI;1.4kb),can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pINDUCER13 (miR-LUP)

Plasmid

#46936

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pHCAG-L2EOP

Plasmid

#51783

Purpose

The oriP/EBV nuclear antigen (EBNA)-1 elements of Epstein-Barr virus is fused in frame with the luciferase in the herpes simplex virus type-1 amplicon vector, provides prolonged transgene expression

Depositor

Paula Lam

Article

Sia et al J Gene Med. 2010 Oct;12(10):848-58. doi:

Inserts

Luciferase-2A-EBNA-1
OriP

Use

Herpes simplex virus type-1 amplicon vector

Tags

Expression

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pGL3-Sox2

Plasmid

#101761

Purpose

Sox2 promoter reporter

Depositor

Yuh-Shan Jou

Article

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

SRY-box 2 promoter (SOX2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT2K-LRL-IRES-Luc2

Plasmid

#109230

Purpose

For Tol2 transposon-mediated stable expression of LRL and IRES-luciferase

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

LRL and Luc2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Availability

Academic Institutions and Nonprofits only

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Luc-Hdm4-3'UTR-F2d5

Plasmid

#64018

Purpose

Luciferase reporter of human Hdm4 3'UTR F2 fragment mutant #5

Depositor

Lin He

Article

Okada et al Genes Dev. 2014 Mar 1;28(5):438-50. doi:

Insert

HDMX (MDM4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)Luc2-Turbo RFP/CMV/WPREdU3

Plasmid

#136959

Purpose

Reporter vector encoding firefly luciferase and TurboRFP fluorescent protein

Depositor

Jakob Reiser

Article

Gills et al Oncotarget. 2018 Aug 24;9(66):32718-3272

Insert

Firefly luciferase and TurboRFP

Use

Lentiviral

Tags

V5 tag

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-eFFly-eGFP

Plasmid

#104834

Purpose

eFFly luciferase for invivo Imaging, eGFP for flourescent detection

Depositor

Irmela Jeremias

Article

Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

Inserts

eFFly
eGFP

Use

Lentiviral and Luciferase; Egfp

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCIneoLuc-TEAD4

Plasmid

#128335

Purpose

Vector for LUMIER assay

Depositor

Yutaka Hata

Article

Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

Insert

TEAD4 (TEAD4 Human)

Use

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Bxb1Donor_WT_ADRB2_LucReporter

Plasmid

#129788

Purpose

encodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

Insert

ADRB2 (ADRB2 Human)

Use

Synthetic Biology

Tags

3x FLAG

Expression

Mammalian

Mutation

Promoter

N/A

Availability

Academic Institutions and Nonprofits only

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Bxb1Donor_W99A_ADRB2_LucReporter

Plasmid

#129789

Purpose

encodes a donor vector for recombination with the Bxb1 recombinase with variant W99A of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

Insert

ADRB2 (ADRB2 Human)

Use

Synthetic Biology

Tags

3x FLAG

Expression

Mammalian

Mutation

W99A

Promoter

N/A

Availability

Academic Institutions and Nonprofits only

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Bxb1Donor_N51L_ADRB2_LucReporter

Plasmid

#129790

Purpose

encodes a donor vector for recombination with the Bxb1 recombinase with variant N51L of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

Insert

ADRB2 (ADRB2 Human)

Use

Synthetic Biology

Tags

3x FLAG

Expression

Mammalian

Mutation

N51L

Promoter

N/A

Availability

Academic Institutions and Nonprofits only

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Bxb1Donor_G315T_ADRB2_LucReporter

Plasmid

#129793

Purpose

encodes a donor vector for recombination with the Bxb1 recombinase with variant G315T of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

Insert

ADRB2 (ADRB2 Human)

Use

Synthetic Biology

Tags

3x FLAG

Expression

Mammalian

Mutation

G315T

Promoter

N/A

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1Donor_G102H_ADRB2_LucReporter

Plasmid

#129794

Purpose

encodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

Insert

ADRB2 (ADRB2 Human)

Use

Synthetic Biology

Tags

3x FLAG

Expression

Mammalian

Mutation

G102H

Promoter

N/A

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO-GAlphai2-RLuc8

Plasmid

#140974

Purpose

Encodes a G alpha subunit (GNAI2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlphai2-Rluc8 (GNAI2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO-GAlphai3-RLuc8

Plasmid

#140975

Purpose

Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlphai3-RLuc8 (GNAI3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO-GAlphaz-RLuc8

Plasmid

#140978

Purpose

Encodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlphaz-Rluc8 (GNAZ Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge